The localization, function and applications of the stress response protein Hsp12p in the yeast Saccharomyces cerevisiae

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dc.contributor.advisorLindsey, George Gen_ZA
dc.contributor.advisorBrandt, Wolf Fen_ZA
dc.contributor.authorKarreman, Robert Janen_ZA
dc.date.accessioned2014-07-30T17:38:36Z
dc.date.available2014-07-30T17:38:36Z
dc.date.issued2006en_ZA
dc.descriptionWord processed copy.
dc.descriptionIncludes bibliographical references (leaves 117-132).
dc.description.abstractSince 1990, the yeast Saccharomyces cerevisiae small heat shock protein Hsp12p, has continuously appeared in data associated with stress responses in this organism. Hsp12p is expressed abundantly in response to a large variety of different stresses, but for many years has eluded researchers as to its function, primarily because the viability of yeast strains lacking HSP12 are unaffected by osmotic stress and heat shock. Subsequent studies indicated that Hsp12p played a role in the adaptation of the cell wall of Saccharomyces cerevisiae to conditions of stress. However, the exact in vivo localization, specific function and mediation of function of Hsp12p had yet to be elucidated. The localization of Hsp12p was determined by fusion to the green fluorescent reporter protein, Gfp2p and a combination of epifluorescent microscopy and confocal imagery. Chemical extraction revealed that Hsp12p was present in the cell wall while fluorescent imagery was not conclusive. This fluorescent Hsp12p construct was later employed in a novel application to sense the stress status of yeast, which bears future promise for use in an industrial setting.en_ZA
dc.identifier.apacitationKarreman, R. J. (2006). <i>The localization, function and applications of the stress response protein Hsp12p in the yeast Saccharomyces cerevisiae</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/4278en_ZA
dc.identifier.chicagocitationKarreman, Robert Jan. <i>"The localization, function and applications of the stress response protein Hsp12p in the yeast Saccharomyces cerevisiae."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2006. http://hdl.handle.net/11427/4278en_ZA
dc.identifier.citationKarreman, R. 2006. The localization, function and applications of the stress response protein Hsp12p in the yeast Saccharomyces cerevisiae. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Karreman, Robert Jan AB - Since 1990, the yeast Saccharomyces cerevisiae small heat shock protein Hsp12p, has continuously appeared in data associated with stress responses in this organism. Hsp12p is expressed abundantly in response to a large variety of different stresses, but for many years has eluded researchers as to its function, primarily because the viability of yeast strains lacking HSP12 are unaffected by osmotic stress and heat shock. Subsequent studies indicated that Hsp12p played a role in the adaptation of the cell wall of Saccharomyces cerevisiae to conditions of stress. However, the exact in vivo localization, specific function and mediation of function of Hsp12p had yet to be elucidated. The localization of Hsp12p was determined by fusion to the green fluorescent reporter protein, Gfp2p and a combination of epifluorescent microscopy and confocal imagery. Chemical extraction revealed that Hsp12p was present in the cell wall while fluorescent imagery was not conclusive. This fluorescent Hsp12p construct was later employed in a novel application to sense the stress status of yeast, which bears future promise for use in an industrial setting. DA - 2006 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2006 T1 - The localization, function and applications of the stress response protein Hsp12p in the yeast Saccharomyces cerevisiae TI - The localization, function and applications of the stress response protein Hsp12p in the yeast Saccharomyces cerevisiae UR - http://hdl.handle.net/11427/4278 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/4278
dc.identifier.vancouvercitationKarreman RJ. The localization, function and applications of the stress response protein Hsp12p in the yeast Saccharomyces cerevisiae. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2006 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/4278en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleThe localization, function and applications of the stress response protein Hsp12p in the yeast Saccharomyces cerevisiaeen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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