Studies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia coli

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dc.contributor.advisorRawlings, Douglas Een_ZA
dc.contributor.authorBrown, Lynn Drydenen_ZA
dc.date.accessioned2016-09-06T14:44:36Z
dc.date.available2016-09-06T14:44:36Z
dc.date.issued1993en_ZA
dc.description.abstractAn atp gene cluster from the extreme acidophile Thiobacillus ferrooxidans ATCC 33020 was cloned by complementation of Escherichia coli unc mutants. Eight different E. coli unc mutants were screened with T. ferrooxidans ATCC 33020 pEcoR251 plasmid and pHC79 cosmid gene banks. The ability of the transformants/transductants to grow on succinate as the sole non-fermentable carbon source was used to select mutants with a functional F₁F₀ ATPsynthase. Many F₁ -complementing plasmids and cosmids were isolated from the four E. coli F₁ unc point mutants screened. No plasmids or cosmids which complemented an E. coli fΔunc strain or any of the three E. coli F₀ mutants screened, were isolated.en_ZA
dc.identifier.apacitationBrown, L. D. (1993). <i>Studies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia coli</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/21697en_ZA
dc.identifier.chicagocitationBrown, Lynn Dryden. <i>"Studies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia coli."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1993. http://hdl.handle.net/11427/21697en_ZA
dc.identifier.citationBrown, L. 1993. Studies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia coli. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis AU - Brown, Lynn Dryden AB - An atp gene cluster from the extreme acidophile Thiobacillus ferrooxidans ATCC 33020 was cloned by complementation of Escherichia coli unc mutants. Eight different E. coli unc mutants were screened with T. ferrooxidans ATCC 33020 pEcoR251 plasmid and pHC79 cosmid gene banks. The ability of the transformants/transductants to grow on succinate as the sole non-fermentable carbon source was used to select mutants with a functional F₁F₀ ATPsynthase. Many F₁ -complementing plasmids and cosmids were isolated from the four E. coli F₁ unc point mutants screened. No plasmids or cosmids which complemented an E. coli fΔunc strain or any of the three E. coli F₀ mutants screened, were isolated. DA - 1993 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1993 T1 - Studies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia coli TI - Studies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia coli UR - http://hdl.handle.net/11427/21697 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21697
dc.identifier.vancouvercitationBrown LD. Studies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia coli. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1993 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/21697en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMicrobiologyen_ZA
dc.titleStudies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia colien_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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