Studies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia coli

Brown, Lynn Dryden

Studies on Thiobacillus ferrooxidans ATCC 33020 ATP genes and gene products, using Escherichia coli

Doctoral Thesis

1993

Publisher

University of Cape Town

Department

Department of Molecular and Cell Biology

Faculty

Faculty of Science

Abstract

An atp gene cluster from the extreme acidophile Thiobacillus ferrooxidans ATCC 33020 was cloned by complementation of Escherichia coli unc mutants. Eight different E. coli unc mutants were screened with T. ferrooxidans ATCC 33020 pEcoR251 plasmid and pHC79 cosmid gene banks. The ability of the transformants/transductants to grow on succinate as the sole non-fermentable carbon source was used to select mutants with a functional F₁F₀ ATPsynthase. Many F₁ -complementing plasmids and cosmids were isolated from the four E. coli F₁ unc point mutants screened. No plasmids or cosmids which complemented an E. coli fΔunc strain or any of the three E. coli F₀ mutants screened, were isolated.

Keywords

Microbiology

Reference:

Collections

PhD / Doctoral

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