The effect of non-biological particulates on microbial cell disruption in a slurry bioreactor

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dc.contributor.advisorHarrison, STLen_ZA
dc.contributor.authorScholtz, Nicola Jeanneen_ZA
dc.date.accessioned2015-06-01T14:10:11Z
dc.date.available2015-06-01T14:10:11Z
dc.date.issued1998en_ZA
dc.descriptionBibliography: leaves [177]-191.en_ZA
dc.description.abstractCell damage from hydrodynamic stress is an important consideration in biological systems since it can result in the growth and function of the cell becoming impaired (Toma et al. 1991, Lilly et al. 1992). In the extreme case of cell damage, cell disruption occurs. This dissertation presents the results of an investigation into the disruption of stationary-phase microbial cells in a stirred tank reactor when agitated in the presence of biologically inert solid particles in the absence of aeration. Applications of biological processes, where cells and solid particles are used, include bead mills, minerals bioprocessing, soil bioremediation and immobilised biocatalysts. An understanding of the rate, extent and mechanisms of cell disruption in these systems will facilitate the design of bioreactors to minimise or maximise microbial cell disruption, depending on the application. The primary objectives were to quantify and model the effect of incompletely and completely suspended solids on the kinetics of cell disruption, as a function of the solids concentration, agitation intensity and impeller flow pattern. Saccharomyces cerevisiae was used as model micro-organism and silica as the solid particles. Modelling the cell disruption enabled its prediction as a function of the operating parameters and further allowed the cell disruption mechanisms to be elucidated. A final objective was to quantify the solids suspension as a function of the operating parameters.en_ZA
dc.identifier.apacitationScholtz, N. J. (1998). <i>The effect of non-biological particulates on microbial cell disruption in a slurry bioreactor</i>. (Thesis). University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Chemical Engineering. Retrieved from http://hdl.handle.net/11427/13043en_ZA
dc.identifier.chicagocitationScholtz, Nicola Jeanne. <i>"The effect of non-biological particulates on microbial cell disruption in a slurry bioreactor."</i> Thesis., University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Chemical Engineering, 1998. http://hdl.handle.net/11427/13043en_ZA
dc.identifier.citationScholtz, N. 1998. The effect of non-biological particulates on microbial cell disruption in a slurry bioreactor. University of Cape Town.en_ZA
dc.identifier.risTY - Thesis / Dissertation AU - Scholtz, Nicola Jeanne AB - Cell damage from hydrodynamic stress is an important consideration in biological systems since it can result in the growth and function of the cell becoming impaired (Toma et al. 1991, Lilly et al. 1992). In the extreme case of cell damage, cell disruption occurs. This dissertation presents the results of an investigation into the disruption of stationary-phase microbial cells in a stirred tank reactor when agitated in the presence of biologically inert solid particles in the absence of aeration. Applications of biological processes, where cells and solid particles are used, include bead mills, minerals bioprocessing, soil bioremediation and immobilised biocatalysts. An understanding of the rate, extent and mechanisms of cell disruption in these systems will facilitate the design of bioreactors to minimise or maximise microbial cell disruption, depending on the application. The primary objectives were to quantify and model the effect of incompletely and completely suspended solids on the kinetics of cell disruption, as a function of the solids concentration, agitation intensity and impeller &#64258;ow pattern. Saccharomyces cerevisiae was used as model micro-organism and silica as the solid particles. Modelling the cell disruption enabled its prediction as a function of the operating parameters and further allowed the cell disruption mechanisms to be elucidated. A final objective was to quantify the solids suspension as a function of the operating parameters. DA - 1998 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1998 T1 - The effect of non-biological particulates on microbial cell disruption in a slurry bioreactor TI - The effect of non-biological particulates on microbial cell disruption in a slurry bioreactor UR - http://hdl.handle.net/11427/13043 ER -en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/13043
dc.identifier.vancouvercitationScholtz NJ. The effect of non-biological particulates on microbial cell disruption in a slurry bioreactor. [Thesis]. University of Cape Town ,Faculty of Engineering & the Built Environment ,Department of Chemical Engineering, 1998 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/13043en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentCentre for Bioprocess Engineering Researchen_ZA
dc.publisher.facultyFaculty of Engineering and the Built Environment
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherBioprocess Engineeringen_ZA
dc.titleThe effect of non-biological particulates on microbial cell disruption in a slurry bioreactoren_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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