Genetic studies on Clostridium acetobutylicum

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dc.contributor.advisorWoods, Daviden_ZA
dc.contributor.advisorReid, Sharon Jen_ZA
dc.contributor.authorAllcock, Errol Ralphen_ZA
dc.date.accessioned2014-12-13T06:22:41Z
dc.date.available2014-12-13T06:22:41Z
dc.date.issued1982en_ZA
dc.descriptionBibliography: leaves 149-181.en_ZA
dc.description.abstractThe aim of this study involved the characterisation of the cellulolytic properties of Clostridium acetobutylicum and the development of a genetic transfer system for this organism. The production of a carboxymethyl cellulose and a cellobiase by C acetobutylicum was demonstrated. In liquid medium the carboxymethyl cellulase was induced by molasses, and it was not repressed by glucose. Optimum carboxymethyl cellulase activity occurred at pH 4.6 and 37°C. Optimum conditions for autolysis and autoplast formation in C. acetobutylicum were defined. Autolysis-deficient mutants which produced less autolysin than the parent strain were isolated. Growth of the P262 strain and the lyt-1 mutant was inhibited by the same concentrations of wall-inhibiting antibiotics.en_ZA
dc.identifier.apacitationAllcock, E. R. (1982). <i>Genetic studies on Clostridium acetobutylicum</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/9977en_ZA
dc.identifier.chicagocitationAllcock, Errol Ralph. <i>"Genetic studies on Clostridium acetobutylicum."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1982. http://hdl.handle.net/11427/9977en_ZA
dc.identifier.citationAllcock, E. 1982. Genetic studies on Clostridium acetobutylicum. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Allcock, Errol Ralph AB - The aim of this study involved the characterisation of the cellulolytic properties of Clostridium acetobutylicum and the development of a genetic transfer system for this organism. The production of a carboxymethyl cellulose and a cellobiase by C acetobutylicum was demonstrated. In liquid medium the carboxymethyl cellulase was induced by molasses, and it was not repressed by glucose. Optimum carboxymethyl cellulase activity occurred at pH 4.6 and 37°C. Optimum conditions for autolysis and autoplast formation in C. acetobutylicum were defined. Autolysis-deficient mutants which produced less autolysin than the parent strain were isolated. Growth of the P262 strain and the lyt-1 mutant was inhibited by the same concentrations of wall-inhibiting antibiotics. DA - 1982 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1982 T1 - Genetic studies on Clostridium acetobutylicum TI - Genetic studies on Clostridium acetobutylicum UR - http://hdl.handle.net/11427/9977 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/9977
dc.identifier.vancouvercitationAllcock ER. Genetic studies on Clostridium acetobutylicum. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1982 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/9977en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMicrobiologyen_ZA
dc.titleGenetic studies on Clostridium acetobutylicumen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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