Genetic studies on Clostridium acetobutylicum
Doctoral Thesis
1982
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University of Cape Town
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Abstract
The aim of this study involved the characterisation of the cellulolytic properties of Clostridium acetobutylicum and the development of a genetic transfer system for this organism. The production of a carboxymethyl cellulose and a cellobiase by C acetobutylicum was demonstrated. In liquid medium the carboxymethyl cellulase was induced by molasses, and it was not repressed by glucose. Optimum carboxymethyl cellulase activity occurred at pH 4.6 and 37°C. Optimum conditions for autolysis and autoplast formation in C. acetobutylicum were defined. Autolysis-deficient mutants which produced less autolysin than the parent strain were isolated. Growth of the P262 strain and the lyt-1 mutant was inhibited by the same concentrations of wall-inhibiting antibiotics.
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Bibliography: leaves 149-181.
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Allcock, E. 1982. Genetic studies on Clostridium acetobutylicum. University of Cape Town.