L-arginine overproduction in Corynebacterium glutamicum ATCC 13032
Doctoral Thesis
2009
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University of Cape Town
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Abstract
Corynebacterium glutamicum is widely used for the commercial production of a variety of amino acids, including L-lysine, L-glutamate and L-threonine. With the exception of Larginine, the biosynthesis and regulation of most of these compounds in this bacterium are relatively well characterised in the literature. The research presented here focuses on improving our understanding of the regulation of L-arginine biosynthesis in C. glutamicum. This was performed with the ultimate goal of creating strains capable of producing L-arginine commercially. A novel gene replacement system was initially used for the directed mutation of the Larginine biosynthetic gene cluster in C. glutamicum ATCC 13032. This was met with limited success, however, and the pK19mobsacB vector was thus adopted for further mutagenesis of this region.
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Includes bibliographical references (p. 155-174).
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Reference:
Theron, G. 2009. L-arginine overproduction in Corynebacterium glutamicum ATCC 13032. University of Cape Town.