Cloning and expression of a modified oryzacystatin inhibitor gene and an investigation of its inhibitory capabilities

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dc.contributor.advisorThomson, Jennifer Annen_ZA
dc.contributor.advisorMaeder, Dennisen_ZA
dc.contributor.authorHaworth, Caroline Joanneen_ZA
dc.date.accessioned2014-11-10T08:59:53Z
dc.date.available2014-11-10T08:59:53Z
dc.date.issued1997en_ZA
dc.descriptionBibliography: leaves 128-146.en_ZA
dc.description.abstractCysteine proteinase inhibitors have shown potential as biocontrol agents for the protection of plants against insect and pathogen attack. With the advent of protein and genetic engineering such inhibitors can now be modified in order to improve their effectiveness. Because cystatins have already been isolated from plants. they provide a good starting point for developing modifications which may improve their function as biocontrol agents. The purpose of this project, therefore, was to design a potentially improved analogue of the rice cysteine proteinase inhibitor, oryzacystatin I, through molecular modelling studies. The gene sequence for this modified protein was then synthesised and expressed for kinetic analysis and insect trial assays. A prediction of the oryzacystatin I (OC I) tertiary structure was made using Biograf software on an Evans and Sutherland workstation. This structure was based on the known structures of stefin B and chicken cystatin ho se co-ordinates are published in the Brookhaven data files. Chicken cystatin is one of the most potent inhibitors of papain in the cystatin superfamily. This is believed to be due, in part, to an increased binding of the cystatin to papain through its amino-terminal region with the residues Leu7 to Gly9 playing a particularly important role.en_ZA
dc.identifier.apacitationHaworth, C. J. (1997). <i>Cloning and expression of a modified oryzacystatin inhibitor gene and an investigation of its inhibitory capabilities</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/9485en_ZA
dc.identifier.chicagocitationHaworth, Caroline Joanne. <i>"Cloning and expression of a modified oryzacystatin inhibitor gene and an investigation of its inhibitory capabilities."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1997. http://hdl.handle.net/11427/9485en_ZA
dc.identifier.citationHaworth, C. 1997. Cloning and expression of a modified oryzacystatin inhibitor gene and an investigation of its inhibitory capabilities. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Haworth, Caroline Joanne AB - Cysteine proteinase inhibitors have shown potential as biocontrol agents for the protection of plants against insect and pathogen attack. With the advent of protein and genetic engineering such inhibitors can now be modified in order to improve their effectiveness. Because cystatins have already been isolated from plants. they provide a good starting point for developing modifications which may improve their function as biocontrol agents. The purpose of this project, therefore, was to design a potentially improved analogue of the rice cysteine proteinase inhibitor, oryzacystatin I, through molecular modelling studies. The gene sequence for this modified protein was then synthesised and expressed for kinetic analysis and insect trial assays. A prediction of the oryzacystatin I (OC I) tertiary structure was made using Biograf software on an Evans and Sutherland workstation. This structure was based on the known structures of stefin B and chicken cystatin ho se co-ordinates are published in the Brookhaven data files. Chicken cystatin is one of the most potent inhibitors of papain in the cystatin superfamily. This is believed to be due, in part, to an increased binding of the cystatin to papain through its amino-terminal region with the residues Leu7 to Gly9 playing a particularly important role. DA - 1997 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1997 T1 - Cloning and expression of a modified oryzacystatin inhibitor gene and an investigation of its inhibitory capabilities TI - Cloning and expression of a modified oryzacystatin inhibitor gene and an investigation of its inhibitory capabilities UR - http://hdl.handle.net/11427/9485 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/9485
dc.identifier.vancouvercitationHaworth CJ. Cloning and expression of a modified oryzacystatin inhibitor gene and an investigation of its inhibitory capabilities. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1997 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/9485en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherBiochemistryen_ZA
dc.titleCloning and expression of a modified oryzacystatin inhibitor gene and an investigation of its inhibitory capabilitiesen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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