Synergism between urinary prothrombin fragment 1 and urine: a comparison of inhibitory activities in stone-prone and stone-free population groups

Simple item page
dc.contributor.authorWebber, Dawn
dc.contributor.authorRodgers, Allen L
dc.contributor.authorSturrock, Edward D
dc.date.accessioned2016-08-05T10:18:38Z
dc.date.available2016-08-05T10:18:38Z
dc.date.issued2002
dc.date.updated2016-08-02T12:48:33Z
dc.description.abstractSouth African blacks rarely form kidney stones compared with whites. This study investigated whether purified urinary prothrombin fragment 1 (UPTF1) derived from blacks is a more potent inhibitor of calcium oxalate crystallisation than that from whites. UPTF1 was purified from the urine of both population groups and their inhibitory activities were compared in a cross-over design in which each protein was tested in ultrafiltered urine from both population groups. Coulter Multisizer, [14C]-oxalate deposition and scanning electron microscopy experiments were used to monitor crystallisation. The study has demonstrated for the first time that UPTF1 promotes nucleation and that inhibitory activity is synergistically dependent upon urine composition. The activity of the whites' UPTF1 was greater than that of the blacks in the whites' urine (e.g. particle size decrease: 31.7% vs. 25.2%), while the blacks' UPTF1 was superior to that of the whites in the blacks' urine (e.g. particle size decrease: 46.5% vs. 32.4%). In addition, when tested in their respective endogenous urines, the blacks' UPTF1 demonstrated superior inhibitory activity on an absolute scale (e.g. particle size decrease: 46.5% vs. 31.7%). Thus, the urine composition of black South Africans may influence their UPTF1 conformation, conferring greater efficacy for inhibition of calcium oxalate crystallisation.en_ZA
dc.identifierhttp://dx.doi.org/10.1515/CCLM.2002.163
dc.identifier.apacitationWebber, D., Rodgers, A. L., & Sturrock, E. D. (2002). Synergism between urinary prothrombin fragment 1 and urine: a comparison of inhibitory activities in stone-prone and stone-free population groups. <i>Clinical Chemistry and Laboratory Medicine: CCLM / FESCC</i>, http://hdl.handle.net/11427/21131en_ZA
dc.identifier.chicagocitationWebber, Dawn, Allen L Rodgers, and Edward D Sturrock "Synergism between urinary prothrombin fragment 1 and urine: a comparison of inhibitory activities in stone-prone and stone-free population groups." <i>Clinical Chemistry and Laboratory Medicine: CCLM / FESCC</i> (2002) http://hdl.handle.net/11427/21131en_ZA
dc.identifier.citationWebber, D., Rodgers, A. L., & Sturrock, E. D. (2002). Synergism between urinary prothrombin fragment 1 and urine: a comparison of inhibitory activities in stone-prone and stone-free population groups. Clinical chemistry and laboratory medicine, 40(9), 930-936.en_ZA
dc.identifier.issn1434-6621en_ZA
dc.identifier.ris TY - Journal Article AU - Webber, Dawn AU - Rodgers, Allen L AU - Sturrock, Edward D AB - South African blacks rarely form kidney stones compared with whites. This study investigated whether purified urinary prothrombin fragment 1 (UPTF1) derived from blacks is a more potent inhibitor of calcium oxalate crystallisation than that from whites. UPTF1 was purified from the urine of both population groups and their inhibitory activities were compared in a cross-over design in which each protein was tested in ultrafiltered urine from both population groups. Coulter Multisizer, [14C]-oxalate deposition and scanning electron microscopy experiments were used to monitor crystallisation. The study has demonstrated for the first time that UPTF1 promotes nucleation and that inhibitory activity is synergistically dependent upon urine composition. The activity of the whites' UPTF1 was greater than that of the blacks in the whites' urine (e.g. particle size decrease: 31.7% vs. 25.2%), while the blacks' UPTF1 was superior to that of the whites in the blacks' urine (e.g. particle size decrease: 46.5% vs. 32.4%). In addition, when tested in their respective endogenous urines, the blacks' UPTF1 demonstrated superior inhibitory activity on an absolute scale (e.g. particle size decrease: 46.5% vs. 31.7%). Thus, the urine composition of black South Africans may influence their UPTF1 conformation, conferring greater efficacy for inhibition of calcium oxalate crystallisation. DA - 2002 DB - OpenUCT DP - University of Cape Town J1 - Clinical Chemistry and Laboratory Medicine: CCLM / FESCC LK - https://open.uct.ac.za PB - University of Cape Town PY - 2002 SM - 1434-6621 T1 - Synergism between urinary prothrombin fragment 1 and urine: a comparison of inhibitory activities in stone-prone and stone-free population groups TI - Synergism between urinary prothrombin fragment 1 and urine: a comparison of inhibitory activities in stone-prone and stone-free population groups UR - http://hdl.handle.net/11427/21131 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21131
dc.identifier.vancouvercitationWebber D, Rodgers AL, Sturrock ED. Synergism between urinary prothrombin fragment 1 and urine: a comparison of inhibitory activities in stone-prone and stone-free population groups. Clinical Chemistry and Laboratory Medicine: CCLM / FESCC. 2002; http://hdl.handle.net/11427/21131.en_ZA
dc.languageengen_ZA
dc.publisherDe Gruyteren_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceClinical Chemistry and Laboratory Medicine: CCLM / FESCCen_ZA
dc.source.urihttp://www.degruyter.com/view/j/cclm.2002.40.issue-9/cclm.2002.163/cclm.2002.163.xml
dc.titleSynergism between urinary prothrombin fragment 1 and urine: a comparison of inhibitory activities in stone-prone and stone-free population groupsen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
Files
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.72 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Journal Articles