Prevalence and trends of Staphylococcus aureus Bacteraemia in hospitalized patients in South Africa, 2010 to 2012: laboratory-based surveillance mapping of antimicrobial resistance and molecular epidemiology

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dc.contributor.authorPerovic, Olgaen_ZA
dc.contributor.authorIyaloo, Samanthaen_ZA
dc.contributor.authorKularatne, Ranminien_ZA
dc.contributor.authorLowman, Warrenen_ZA
dc.contributor.authorBosman, Nomaen_ZA
dc.contributor.authorWadula, Jeannetteen_ZA
dc.contributor.authorSeetharam, Sharonaen_ZA
dc.contributor.authorDuse, Adrianoen_ZA
dc.contributor.authorMbelle, Nontombien_ZA
dc.contributor.authorBamford, Colleenen_ZA
dc.contributor.authorDawood, Halimaen_ZA
dc.contributor.authorMahabeer, Yesholataen_ZA
dc.contributor.authorBhola, Prathnaen_ZA
dc.contributor.authorAbrahams, Shareefen_ZA
dc.contributor.authorSingh-Moodley, Ashikaen_ZA
dc.date.accessioned2016-02-10T14:46:29Z
dc.date.available2016-02-10T14:46:29Z
dc.date.issued2015en_ZA
dc.description.abstractIntroduction We aimed to obtain an in-depth understanding on recent antimicrobial resistance trends and molecular epidemiology trends of S . aureus bacteraemia (SAB). METHODS: Thirteen academic centres in South Africa were included from June 2010 until July 2012. S . aureus susceptibility testing was performed on the MicroScan Walkaway. Real-time PCR using the LightCycler 480 II was done for mec A and nuc . SCC mec and spa -typing were finalized with conventional PCR. We selected one isolate per common spa type per province for multilocus sequence typing (MLST). RESULTS: S . aureus from 2709 patients were included, and 1231 (46%) were resistant to methicillin, with a significant decline over the three-year period (p-value = 0.003). Geographical distribution of MRSA was significantly higher in Gauteng compared to the other provinces (P<0.001). Children <5 years were significantly associated with MRSA with higher rates compared to all other age groups (P = 0.01). The most prevalent SCC mec type was SCC mec type III (531 [41%]) followed by type IV (402 [31%]). Spa -typing discovered 47 different spa -types. The five (87%) most common spa- types were t037, t1257, t045, t064 and t012. Based on MLST, the commonest was ST612 clonal complex (CC8) (n = 7) followed by ST5 (CC5) (n = 4), ST36 (CC30) (n = 4) and ST239 (CC8) (n = 3). CONCLUSIONS: MRSA rate is high in South Africa. Majority of the isolates were classified as SCC mec type III (41%) and type IV (31%), which are typically associated with hospital and community- acquired infections, respectively. Overall, this study reveals the presence of a variety of hospital-acquired MRSA clones in South Africa dominance of few clones, spa 037 and 1257. Monitoring trends in resistance and molecular typing is recommended to detect changing epidemiological trends in AMR patterns of SAB.en_ZA
dc.identifier.apacitationPerovic, O., Iyaloo, S., Kularatne, R., Lowman, W., Bosman, N., Wadula, J., ... Singh-Moodley, A. (2015). Prevalence and trends of Staphylococcus aureus Bacteraemia in hospitalized patients in South Africa, 2010 to 2012: laboratory-based surveillance mapping of antimicrobial resistance and molecular epidemiology. <i>PLoS One</i>, http://hdl.handle.net/11427/16948en_ZA
dc.identifier.chicagocitationPerovic, Olga, Samantha Iyaloo, Ranmini Kularatne, Warren Lowman, Noma Bosman, Jeannette Wadula, Sharona Seetharam, et al "Prevalence and trends of Staphylococcus aureus Bacteraemia in hospitalized patients in South Africa, 2010 to 2012: laboratory-based surveillance mapping of antimicrobial resistance and molecular epidemiology." <i>PLoS One</i> (2015) http://hdl.handle.net/11427/16948en_ZA
dc.identifier.citationPerovic, O., Iyaloo, S., Kularatne, R., Lowman, W., Bosman, N., Wadula, J., ... & Dawood, H. (2015). Prevalence and trends of Staphylococcus aureus Bacteraemia in hospitalized patients in South Africa, 2010 to 2012: laboratory-based surveillance mapping of antimicrobial resistance and molecular epidemiology. PloS one, 10(12), e0145429. doi:10.1371/journal.pone.0145429en_ZA
dc.identifier.ris TY - Journal Article AU - Perovic, Olga AU - Iyaloo, Samantha AU - Kularatne, Ranmini AU - Lowman, Warren AU - Bosman, Noma AU - Wadula, Jeannette AU - Seetharam, Sharona AU - Duse, Adriano AU - Mbelle, Nontombi AU - Bamford, Colleen AU - Dawood, Halima AU - Mahabeer, Yesholata AU - Bhola, Prathna AU - Abrahams, Shareef AU - Singh-Moodley, Ashika AB - Introduction We aimed to obtain an in-depth understanding on recent antimicrobial resistance trends and molecular epidemiology trends of S . aureus bacteraemia (SAB). METHODS: Thirteen academic centres in South Africa were included from June 2010 until July 2012. S . aureus susceptibility testing was performed on the MicroScan Walkaway. Real-time PCR using the LightCycler 480 II was done for mec A and nuc . SCC mec and spa -typing were finalized with conventional PCR. We selected one isolate per common spa type per province for multilocus sequence typing (MLST). RESULTS: S . aureus from 2709 patients were included, and 1231 (46%) were resistant to methicillin, with a significant decline over the three-year period (p-value = 0.003). Geographical distribution of MRSA was significantly higher in Gauteng compared to the other provinces (P<0.001). Children <5 years were significantly associated with MRSA with higher rates compared to all other age groups (P = 0.01). The most prevalent SCC mec type was SCC mec type III (531 [41%]) followed by type IV (402 [31%]). Spa -typing discovered 47 different spa -types. The five (87%) most common spa- types were t037, t1257, t045, t064 and t012. Based on MLST, the commonest was ST612 clonal complex (CC8) (n = 7) followed by ST5 (CC5) (n = 4), ST36 (CC30) (n = 4) and ST239 (CC8) (n = 3). CONCLUSIONS: MRSA rate is high in South Africa. Majority of the isolates were classified as SCC mec type III (41%) and type IV (31%), which are typically associated with hospital and community- acquired infections, respectively. Overall, this study reveals the presence of a variety of hospital-acquired MRSA clones in South Africa dominance of few clones, spa 037 and 1257. Monitoring trends in resistance and molecular typing is recommended to detect changing epidemiological trends in AMR patterns of SAB. DA - 2015 DB - OpenUCT DO - 10.1371/journal.pone.0145429 DP - University of Cape Town J1 - PLoS One LK - https://open.uct.ac.za PB - University of Cape Town PY - 2015 T1 - Prevalence and trends of Staphylococcus aureus Bacteraemia in hospitalized patients in South Africa, 2010 to 2012: laboratory-based surveillance mapping of antimicrobial resistance and molecular epidemiology TI - Prevalence and trends of Staphylococcus aureus Bacteraemia in hospitalized patients in South Africa, 2010 to 2012: laboratory-based surveillance mapping of antimicrobial resistance and molecular epidemiology UR - http://hdl.handle.net/11427/16948 ER - en_ZA
dc.identifier.urihttp://dx.doi.org/10.1371/journal.pone.0145429en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/16948
dc.identifier.vancouvercitationPerovic O, Iyaloo S, Kularatne R, Lowman W, Bosman N, Wadula J, et al. Prevalence and trends of Staphylococcus aureus Bacteraemia in hospitalized patients in South Africa, 2010 to 2012: laboratory-based surveillance mapping of antimicrobial resistance and molecular epidemiology. PLoS One. 2015; http://hdl.handle.net/11427/16948.en_ZA
dc.language.isoengen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.publisher.departmentInstitute of Infectious Disease and Molecular Medicineen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_ZA
dc.rights.holder© 2015 Perovic et alen_ZA
dc.rights.urihttp://creativecommons.org/licenses/by/4.0en_ZA
dc.sourcePLoS Oneen_ZA
dc.source.urihttp://journals.plos.org/plosoneen_ZA
dc.subject.otherMethicillin-resistant Staphylococcus aureusen_ZA
dc.subject.otherAntimicrobial resistanceen_ZA
dc.subject.otherSouth Africaen_ZA
dc.subject.otherStaphylococcus aureusen_ZA
dc.subject.otherPolymerase chain reactionen_ZA
dc.subject.otherCloningen_ZA
dc.subject.otherAntimicrobialsen_ZA
dc.subject.otherNosocomial infectionsen_ZA
dc.titlePrevalence and trends of Staphylococcus aureus Bacteraemia in hospitalized patients in South Africa, 2010 to 2012: laboratory-based surveillance mapping of antimicrobial resistance and molecular epidemiologyen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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