The OP27 cell line as a model system to study the effects of FGF-2 in olfactory neuronal development

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dc.contributor.authorShoko, Aubrey Thembaen_ZA
dc.date.accessioned2014-11-03T08:14:22Z
dc.date.available2014-11-03T08:14:22Z
dc.date.issued2004en_ZA
dc.descriptionIncludes bibliographical references.en_ZA
dc.description.abstractDue to its unique capacity to regenerate continously, the olfactory neuroepithelium serves as an excellent model system for investigating the molecular and cellular mechanisms involved in neurogenesis. The OP27 cell line (generated by infecting embryonic mouse olfactory placodes with a retrovirus carrying the temperature-sensitive alleles of the SV 40 large T antigen) was used as an in vitro system to test the effects of fibroblast growth factor-2 (FGF-2) in directing olfactory neurogenesis. The OP27 cells proliferate under the control of the retrovirus at the permissive temperature (33°C). When shifted to the non-permissive temperature (39°C) the SV40 large T antigen is inactivated and the cells stop dividing, thereby allowing one to study the effects of growth factors on these cells. Although FGF-2 also plays an important role in regulating the proliferation of neural progenitors, the main focus in this study was its effect on neuronal differentiation.en_ZA
dc.identifier.apacitationShoko, A. T. (2004). <i>The OP27 cell line as a model system to study the effects of FGF-2 in olfactory neuronal development</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/9028en_ZA
dc.identifier.chicagocitationShoko, Aubrey Themba. <i>"The OP27 cell line as a model system to study the effects of FGF-2 in olfactory neuronal development."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2004. http://hdl.handle.net/11427/9028en_ZA
dc.identifier.citationShoko, A. 2004. The OP27 cell line as a model system to study the effects of FGF-2 in olfactory neuronal development. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Shoko, Aubrey Themba AB - Due to its unique capacity to regenerate continously, the olfactory neuroepithelium serves as an excellent model system for investigating the molecular and cellular mechanisms involved in neurogenesis. The OP27 cell line (generated by infecting embryonic mouse olfactory placodes with a retrovirus carrying the temperature-sensitive alleles of the SV 40 large T antigen) was used as an in vitro system to test the effects of fibroblast growth factor-2 (FGF-2) in directing olfactory neurogenesis. The OP27 cells proliferate under the control of the retrovirus at the permissive temperature (33°C). When shifted to the non-permissive temperature (39°C) the SV40 large T antigen is inactivated and the cells stop dividing, thereby allowing one to study the effects of growth factors on these cells. Although FGF-2 also plays an important role in regulating the proliferation of neural progenitors, the main focus in this study was its effect on neuronal differentiation. DA - 2004 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2004 T1 - The OP27 cell line as a model system to study the effects of FGF-2 in olfactory neuronal development TI - The OP27 cell line as a model system to study the effects of FGF-2 in olfactory neuronal development UR - http://hdl.handle.net/11427/9028 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/9028
dc.identifier.vancouvercitationShoko AT. The OP27 cell line as a model system to study the effects of FGF-2 in olfactory neuronal development. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2004 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/9028en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleThe OP27 cell line as a model system to study the effects of FGF-2 in olfactory neuronal developmenten_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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