Correlates of risk of TB disease in infants with differential response to BCG vaccination

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dc.contributor.advisorHanekom, Willem Aen_ZA
dc.contributor.advisorNemes, Elisaen_ZA
dc.contributor.advisorScriba, Thomas Jen_ZA
dc.contributor.authorNjikan, Samuel Ayabaen_ZA
dc.date.accessioned2015-05-26T14:16:58Z
dc.date.available2015-05-26T14:16:58Z
dc.date.issued2014en_ZA
dc.descriptionIncludes bibliographical references.en_ZA
dc.description.abstractStudying prospective immune correlates of risk of TB disease following BCG vaccination is an important first step towards determining correlates of protection against TB, which can be identified only in a placebo-controlled randomized controlled trial (RCT) of an effective vaccine. To study correlates of risk of TB disease, we collected and stored blood from healthy 10-week old infants vaccinated with BCG at birth. During two years of follow up, infants who developed lung TB were defined as cases, while those who did not develop TB disease were defined as controls. We measured Th1/Th17 cytokine production by BCG-specific T cells, release of pro- and anti-inflammatory mediators, cytotoxic T cell potential and proliferation in response to BCG as potential correlates of risk of TB disease but none of these outcomes were different between cases and controls. However, transcriptional profiling of PBMC revealed two clusters of infants and interestingly, the gene expression profiles from cases and controls in the two clusters were in opposite directions. Based on this, we hypothesised that analysing the two clusters of infants separately will allow discovery of correlates of risk of TB, which were absent when clustering was not taken into account.en_ZA
dc.identifier.apacitationNjikan, S. A. (2014). <i>Correlates of risk of TB disease in infants with differential response to BCG vaccination</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Department of Paediatrics and Child Health. Retrieved from http://hdl.handle.net/11427/12873en_ZA
dc.identifier.chicagocitationNjikan, Samuel Ayaba. <i>"Correlates of risk of TB disease in infants with differential response to BCG vaccination."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Department of Paediatrics and Child Health, 2014. http://hdl.handle.net/11427/12873en_ZA
dc.identifier.citationNjikan, S. 2014. Correlates of risk of TB disease in infants with differential response to BCG vaccination. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Njikan, Samuel Ayaba AB - Studying prospective immune correlates of risk of TB disease following BCG vaccination is an important first step towards determining correlates of protection against TB, which can be identified only in a placebo-controlled randomized controlled trial (RCT) of an effective vaccine. To study correlates of risk of TB disease, we collected and stored blood from healthy 10-week old infants vaccinated with BCG at birth. During two years of follow up, infants who developed lung TB were defined as cases, while those who did not develop TB disease were defined as controls. We measured Th1/Th17 cytokine production by BCG-specific T cells, release of pro- and anti-inflammatory mediators, cytotoxic T cell potential and proliferation in response to BCG as potential correlates of risk of TB disease but none of these outcomes were different between cases and controls. However, transcriptional profiling of PBMC revealed two clusters of infants and interestingly, the gene expression profiles from cases and controls in the two clusters were in opposite directions. Based on this, we hypothesised that analysing the two clusters of infants separately will allow discovery of correlates of risk of TB, which were absent when clustering was not taken into account. DA - 2014 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2014 T1 - Correlates of risk of TB disease in infants with differential response to BCG vaccination TI - Correlates of risk of TB disease in infants with differential response to BCG vaccination UR - http://hdl.handle.net/11427/12873 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/12873
dc.identifier.vancouvercitationNjikan SA. Correlates of risk of TB disease in infants with differential response to BCG vaccination. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Department of Paediatrics and Child Health, 2014 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/12873en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Paediatrics and Child Healthen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherClinical Science & Immunologyen_ZA
dc.titleCorrelates of risk of TB disease in infants with differential response to BCG vaccinationen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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