Isolation and characterisation of antibiotic-producing marine actinomycetes

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dc.contributor.advisorMeyers, Paulen_ZA
dc.contributor.authorPorter, Donovan Stuarten_ZA
dc.date.accessioned2014-10-18T05:54:58Z
dc.date.available2014-10-18T05:54:58Z
dc.date.issued2003en_ZA
dc.descriptionBibliography: leaves 94-102.en_ZA
dc.description.abstractResistance to antibiotics poses a serious threat to healthcare and new drugs are needed. This is especially true for tuberculosis (TB), which is at epidemic levels in South Africa. Multidrug-resistance in Mycobacterium tuberculosis makes TB more difficult and expensive to treat and increases mortality rates. The surfaces of 12 seaweed species found in South African coastal waters were screened for the presence of antibiotic-producing actinomycetes. Of the 67 strains isolated, 26 exhibited antibacterial activity against Mycobacterium aurum A+ and for Enterococcus faecium VanA. These actinomycete strains were physiologically characterised. Three strains showing very strong antibacterial activity were further characterised by the use of chemical taxonomy, DNA sequencing and scanning electron microscopy and were shown to belong to the genus Streptomyces. A strain not showing activity was shown by the same methods to belong to the genus Micromonospora. Partial purification of the active compounds was carried out on the three strains exhibiting strong antibacterial activity. All were shown to produce moderately to highly polar compounds.en_ZA
dc.identifier.apacitationPorter, D. S. (2003). <i>Isolation and characterisation of antibiotic-producing marine actinomycetes</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/8591en_ZA
dc.identifier.chicagocitationPorter, Donovan Stuart. <i>"Isolation and characterisation of antibiotic-producing marine actinomycetes."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2003. http://hdl.handle.net/11427/8591en_ZA
dc.identifier.citationPorter, D. 2003. Isolation and characterisation of antibiotic-producing marine actinomycetes. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Porter, Donovan Stuart AB - Resistance to antibiotics poses a serious threat to healthcare and new drugs are needed. This is especially true for tuberculosis (TB), which is at epidemic levels in South Africa. Multidrug-resistance in Mycobacterium tuberculosis makes TB more difficult and expensive to treat and increases mortality rates. The surfaces of 12 seaweed species found in South African coastal waters were screened for the presence of antibiotic-producing actinomycetes. Of the 67 strains isolated, 26 exhibited antibacterial activity against Mycobacterium aurum A+ and for Enterococcus faecium VanA. These actinomycete strains were physiologically characterised. Three strains showing very strong antibacterial activity were further characterised by the use of chemical taxonomy, DNA sequencing and scanning electron microscopy and were shown to belong to the genus Streptomyces. A strain not showing activity was shown by the same methods to belong to the genus Micromonospora. Partial purification of the active compounds was carried out on the three strains exhibiting strong antibacterial activity. All were shown to produce moderately to highly polar compounds. DA - 2003 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2003 T1 - Isolation and characterisation of antibiotic-producing marine actinomycetes TI - Isolation and characterisation of antibiotic-producing marine actinomycetes UR - http://hdl.handle.net/11427/8591 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/8591
dc.identifier.vancouvercitationPorter DS. Isolation and characterisation of antibiotic-producing marine actinomycetes. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2003 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/8591en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleIsolation and characterisation of antibiotic-producing marine actinomycetesen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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