Nitrogen metabolism and butanol production by South African clostridium beijerinckii and clostridium saccharobutylicum strains

Simple item page
dc.contributor.advisorReid, Sharon Jen_ZA
dc.contributor.authorReeve, Byron William Patricken_ZA
dc.date.accessioned2015-05-28T04:11:15Z
dc.date.available2015-05-28T04:11:15Z
dc.date.issued2014en_ZA
dc.descriptionIncludes bibliographical references.en_ZA
dc.description.abstractThe acetone- butanol-ethanol (ABE) fermentation was one of the first fermentation processes to be industrialized on a large scale, and the dominant product, butanol is particularly significant due to its potential as a modern day fuel additive or fuel extender in the petrochemical industry. A collection of 19 solventogenic Clostridium beijerinckii and 11 Clostridium saccharobutylicum strains isolated from the National Chemical Products (NCP) ABE fermentation plant in Germiston, South Africa, were classed according to species by a quick species-specific colony PCR and by rifampicin screening methods respectively. The speciesspecific PCR aims to provide a rapid means of assessing any contamination of an ABE batch fermentation by differentiating between C. saccharobutylicum and C. beijerinckii species. Random Amplification of Polymorphic DNA (RAPD) analysis generated four C. beijerinckii and two C. saccharobutylicum strain groups respectively. Multilocus Sequence Typing (MLST) was developed for a smaller selection of strains and showed a further two strain groups within the NCP C. beijerinckii strains and three groups within the C. saccharobutylicum strains.en_ZA
dc.identifier.apacitationReeve, B. W. P. (2014). <i>Nitrogen metabolism and butanol production by South African clostridium beijerinckii and clostridium saccharobutylicum strains</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/12946en_ZA
dc.identifier.chicagocitationReeve, Byron William Patrick. <i>"Nitrogen metabolism and butanol production by South African clostridium beijerinckii and clostridium saccharobutylicum strains."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2014. http://hdl.handle.net/11427/12946en_ZA
dc.identifier.citationReeve, B. 2014. Nitrogen metabolism and butanol production by South African clostridium beijerinckii and clostridium saccharobutylicum strains. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Reeve, Byron William Patrick AB - The acetone- butanol-ethanol (ABE) fermentation was one of the first fermentation processes to be industrialized on a large scale, and the dominant product, butanol is particularly significant due to its potential as a modern day fuel additive or fuel extender in the petrochemical industry. A collection of 19 solventogenic Clostridium beijerinckii and 11 Clostridium saccharobutylicum strains isolated from the National Chemical Products (NCP) ABE fermentation plant in Germiston, South Africa, were classed according to species by a quick species-specific colony PCR and by rifampicin screening methods respectively. The speciesspecific PCR aims to provide a rapid means of assessing any contamination of an ABE batch fermentation by differentiating between C. saccharobutylicum and C. beijerinckii species. Random Amplification of Polymorphic DNA (RAPD) analysis generated four C. beijerinckii and two C. saccharobutylicum strain groups respectively. Multilocus Sequence Typing (MLST) was developed for a smaller selection of strains and showed a further two strain groups within the NCP C. beijerinckii strains and three groups within the C. saccharobutylicum strains. DA - 2014 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2014 T1 - Nitrogen metabolism and butanol production by South African clostridium beijerinckii and clostridium saccharobutylicum strains TI - Nitrogen metabolism and butanol production by South African clostridium beijerinckii and clostridium saccharobutylicum strains UR - http://hdl.handle.net/11427/12946 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/12946
dc.identifier.vancouvercitationReeve BWP. Nitrogen metabolism and butanol production by South African clostridium beijerinckii and clostridium saccharobutylicum strains. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2014 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/12946en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMolecular and Cell Biologyen_ZA
dc.titleNitrogen metabolism and butanol production by South African clostridium beijerinckii and clostridium saccharobutylicum strainsen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
thesis_sci_2014_reeve_b.pdf
Size:
5.07 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
PhD / Doctoral