Cloning and molecular characterisation of four alginate lyase genes from Vibrio midae SY9 : an enteric bacterium from the abalone Haliotis midae

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dc.contributor.advisorCoyne, Vernonen_ZA
dc.contributor.authorCross, Bronwenen_ZA
dc.date.accessioned2014-07-30T17:36:18Z
dc.date.available2014-07-30T17:36:18Z
dc.date.issued2010en_ZA
dc.descriptionIncludes abstract.
dc.descriptionIncludes bibliographical references (leaves 241-268).
dc.description.abstractAlginate is a linear, un-branched polysaccharide of (1-4)-linked -D-mannuronate (M) and its C-5 epimer, -L-guluronate acid (G). These uronic acids are arranged in three different block types in the alginate polymer; poly-M, poly-G or poly-MG. Alginate lyases are enzymes that utilize a -elimination reaction to depolymerise the alginate polymer resulting in cleavage of the (1-4)-O-glycosidic linkage between monomers and the formation of an unsaturated uronic acid at the new non-reducing terminus. Alginate lyases have been isolated from a wide range of sources including marine invertebrates and marine bacteria, which often produce more than one alginate lyase enzyme. Haliotis midae is the commercially important abalone species found along the South African coast. Over-fishing and poaching of this species has led to a depletion of the naturally occurring populations and closure of the recreational and commercial fisheries. Abalone farming was initiated and has rapidly increased in response to the increasing demand for this delicacy. However, there are many problems associated with abalone aquaculture, the most significant being disease and the slow growth rates of the animals. The use of probiotics in abalone aquaculture is a potential solution to both of these problems.en_ZA
dc.identifier.apacitationCross, B. (2010). <i>Cloning and molecular characterisation of four alginate lyase genes from Vibrio midae SY9 : an enteric bacterium from the abalone Haliotis midae</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/4253en_ZA
dc.identifier.chicagocitationCross, Bronwen. <i>"Cloning and molecular characterisation of four alginate lyase genes from Vibrio midae SY9 : an enteric bacterium from the abalone Haliotis midae."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2010. http://hdl.handle.net/11427/4253en_ZA
dc.identifier.citationCross, B. 2010. Cloning and molecular characterisation of four alginate lyase genes from Vibrio midae SY9 : an enteric bacterium from the abalone Haliotis midae. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Cross, Bronwen AB - Alginate is a linear, un-branched polysaccharide of (1-4)-linked -D-mannuronate (M) and its C-5 epimer, -L-guluronate acid (G). These uronic acids are arranged in three different block types in the alginate polymer; poly-M, poly-G or poly-MG. Alginate lyases are enzymes that utilize a -elimination reaction to depolymerise the alginate polymer resulting in cleavage of the (1-4)-O-glycosidic linkage between monomers and the formation of an unsaturated uronic acid at the new non-reducing terminus. Alginate lyases have been isolated from a wide range of sources including marine invertebrates and marine bacteria, which often produce more than one alginate lyase enzyme. Haliotis midae is the commercially important abalone species found along the South African coast. Over-fishing and poaching of this species has led to a depletion of the naturally occurring populations and closure of the recreational and commercial fisheries. Abalone farming was initiated and has rapidly increased in response to the increasing demand for this delicacy. However, there are many problems associated with abalone aquaculture, the most significant being disease and the slow growth rates of the animals. The use of probiotics in abalone aquaculture is a potential solution to both of these problems. DA - 2010 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2010 T1 - Cloning and molecular characterisation of four alginate lyase genes from Vibrio midae SY9 : an enteric bacterium from the abalone Haliotis midae TI - Cloning and molecular characterisation of four alginate lyase genes from Vibrio midae SY9 : an enteric bacterium from the abalone Haliotis midae UR - http://hdl.handle.net/11427/4253 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/4253
dc.identifier.vancouvercitationCross B. Cloning and molecular characterisation of four alginate lyase genes from Vibrio midae SY9 : an enteric bacterium from the abalone Haliotis midae. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2010 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/4253en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleCloning and molecular characterisation of four alginate lyase genes from Vibrio midae SY9 : an enteric bacterium from the abalone Haliotis midaeen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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