Studies on the regulation of extracellular collagenase production by vibrio alginolyticus

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dc.contributor.advisorWoods, David Ren_ZA
dc.contributor.advisorRobb, Frank Ten_ZA
dc.contributor.authorReid, Graham Charltonen_ZA
dc.date.accessioned2014-12-13T06:22:46Z
dc.date.available2014-12-13T06:22:46Z
dc.date.issued1981en_ZA
dc.descriptionBibliography: leaves 117-133.en_ZA
dc.description.abstractVibrio alginolyticus synthesized extracellular collagenase in a highly aerated peptone medium at the late-exponential and early-stationary phases of growth. Collagenase synthesis was subject to end-product repression and was repressed by various amino acids and ammonium ions. Glutamine caused severe repression of collagenase production. Collagenase synthesis was sensitive to catabolite repression by glucose and a number of carbon sources. Cyclic AMP, dibutyryl cyclic AMP and cyclic GMP did not relieve catabolite repression. Glucose and 2-deoxyglucose caused a severe transient repression. No intracellular preformed collagenase was detected and collagenase production ceased when induced cells were washed and resuspended in buffer.Trypsin and a-chymotrypsin had no effect on collagenase production by cells or sphaeroplasts. The inducers of collagenase production in peptone were shown to have abroad molecular weight range between 1, 000 and 60,000. The peptone inducers supported slow growth of V. alginolyticus when supplied as the sole nitrogen source in minimal medium. Digestion of the peptone inducers with purified V. alginolyticus collagenase resulted in a decrease in their inducing ability,whereas digestion with trypsin or a-chymotrypsin did not. Peptone acted as an inhibitor of collagenase.en_ZA
dc.identifier.apacitationReid, G. C. (1981). <i>Studies on the regulation of extracellular collagenase production by vibrio alginolyticus</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/9978en_ZA
dc.identifier.chicagocitationReid, Graham Charlton. <i>"Studies on the regulation of extracellular collagenase production by vibrio alginolyticus."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1981. http://hdl.handle.net/11427/9978en_ZA
dc.identifier.citationReid, G. 1981. Studies on the regulation of extracellular collagenase production by vibrio alginolyticus. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Reid, Graham Charlton AB - Vibrio alginolyticus synthesized extracellular collagenase in a highly aerated peptone medium at the late-exponential and early-stationary phases of growth. Collagenase synthesis was subject to end-product repression and was repressed by various amino acids and ammonium ions. Glutamine caused severe repression of collagenase production. Collagenase synthesis was sensitive to catabolite repression by glucose and a number of carbon sources. Cyclic AMP, dibutyryl cyclic AMP and cyclic GMP did not relieve catabolite repression. Glucose and 2-deoxyglucose caused a severe transient repression. No intracellular preformed collagenase was detected and collagenase production ceased when induced cells were washed and resuspended in buffer.Trypsin and a-chymotrypsin had no effect on collagenase production by cells or sphaeroplasts. The inducers of collagenase production in peptone were shown to have abroad molecular weight range between 1, 000 and 60,000. The peptone inducers supported slow growth of V. alginolyticus when supplied as the sole nitrogen source in minimal medium. Digestion of the peptone inducers with purified V. alginolyticus collagenase resulted in a decrease in their inducing ability,whereas digestion with trypsin or a-chymotrypsin did not. Peptone acted as an inhibitor of collagenase. DA - 1981 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1981 T1 - Studies on the regulation of extracellular collagenase production by vibrio alginolyticus TI - Studies on the regulation of extracellular collagenase production by vibrio alginolyticus UR - http://hdl.handle.net/11427/9978 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/9978
dc.identifier.vancouvercitationReid GC. Studies on the regulation of extracellular collagenase production by vibrio alginolyticus. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1981 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/9978en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMicrobiologyen_ZA
dc.titleStudies on the regulation of extracellular collagenase production by vibrio alginolyticusen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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