The lysis of Gram-negative Alcaligenes eutrophus by enzymes from Cytophaga

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dc.contributor.authorHarrison, Susan T L
dc.contributor.authorChase, Howard A
dc.contributor.authorDennis, John S
dc.date.accessioned2016-08-19T11:14:06Z
dc.date.available2016-08-19T11:14:06Z
dc.date.issued1991
dc.date.updated2016-08-16T14:10:45Z
dc.description.abstractThe use of Cytophaga lysing enzymes was investigated for the liberation of poly-~- hydroxybutyrate (PHB) granules from the Gram-negative bacterium AlcaIigenes eutrophus. Complete cell lysis was approached within a 60 minute period. Contrary to previous findings for the lysis of Gram-negative bacteria, prior removal of the outer membrane was not essential for enzymic lysis. The destabilisation of the outer membrane by the removal of divalent cations resulted in no significant improvement in the disruption process.en_ZA
dc.identifierhttp://dx.doi.org/DOI: 10.1007/BF00159982
dc.identifier.apacitationHarrison, S. T. L., Chase, H. A., & Dennis, J. S. (1991). The lysis of Gram-negative Alcaligenes eutrophus by enzymes from Cytophaga. <i>Biotechnology Techniques</i>, http://hdl.handle.net/11427/21353en_ZA
dc.identifier.chicagocitationHarrison, Susan T L, Howard A Chase, and John S Dennis "The lysis of Gram-negative Alcaligenes eutrophus by enzymes from Cytophaga." <i>Biotechnology Techniques</i> (1991) http://hdl.handle.net/11427/21353en_ZA
dc.identifier.citationHarrison, S. T., Chase, H. A., & Dennis, J. S. (1991). The lysis of Gram-negative Alcaligenes eutrophus by enzymes from Cytophaga. Biotechnology Techniques, 5(2), 115-120.en_ZA
dc.identifier.issn0951-208Xen_ZA
dc.identifier.ris TY - Journal Article AU - Harrison, Susan T L AU - Chase, Howard A AU - Dennis, John S AB - The use of Cytophaga lysing enzymes was investigated for the liberation of poly-~- hydroxybutyrate (PHB) granules from the Gram-negative bacterium AlcaIigenes eutrophus. Complete cell lysis was approached within a 60 minute period. Contrary to previous findings for the lysis of Gram-negative bacteria, prior removal of the outer membrane was not essential for enzymic lysis. The destabilisation of the outer membrane by the removal of divalent cations resulted in no significant improvement in the disruption process. DA - 1991 DB - OpenUCT DP - University of Cape Town J1 - Biotechnology Techniques LK - https://open.uct.ac.za PB - University of Cape Town PY - 1991 SM - 0951-208X T1 - The lysis of Gram-negative Alcaligenes eutrophus by enzymes from Cytophaga TI - The lysis of Gram-negative Alcaligenes eutrophus by enzymes from Cytophaga UR - http://hdl.handle.net/11427/21353 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21353
dc.identifier.vancouvercitationHarrison STL, Chase HA, Dennis JS. The lysis of Gram-negative Alcaligenes eutrophus by enzymes from Cytophaga. Biotechnology Techniques. 1991; http://hdl.handle.net/11427/21353.en_ZA
dc.languageengen_ZA
dc.publisherSpringer Verlagen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.sourceBiotechnology Techniquesen_ZA
dc.source.urihttp://link.springer.com/journal/10542
dc.titleThe lysis of Gram-negative Alcaligenes eutrophus by enzymes from Cytophagaen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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