The lysis of Gram-negative Alcaligenes eutrophus by enzymes from Cytophaga
Journal Article
1991
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Biotechnology Techniques
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Springer Verlag
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University of Cape Town
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Abstract
The use of Cytophaga lysing enzymes was investigated for the liberation of poly-~- hydroxybutyrate (PHB) granules from the Gram-negative bacterium AlcaIigenes eutrophus. Complete cell lysis was approached within a 60 minute period. Contrary to previous findings for the lysis of Gram-negative bacteria, prior removal of the outer membrane was not essential for enzymic lysis. The destabilisation of the outer membrane by the removal of divalent cations resulted in no significant improvement in the disruption process.
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Reference:
Harrison, S. T., Chase, H. A., & Dennis, J. S. (1991). The lysis of Gram-negative Alcaligenes eutrophus by enzymes from Cytophaga. Biotechnology Techniques, 5(2), 115-120.