Cloning, expression, purification and drug targeting of Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT)

Doctoral Thesis

2005

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http://hdl.handle.net/11427/2696
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thesis_hsf_2005_mbewe_b.pdf (9.52 MB)
Authors
Mbewe, Boniface
Supervisors
Mcintosh, David B
Chibale, Kelly
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University of Cape Town

Department
Division of Chemical Pathology
Faculty
Faculty of Health Sciences
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Abstract
The research concerns sub-cloning the gene for HGXPRT from Plasmodium falciparum from a vector with a His-tag facility to one without, expression of the protein in E. coli, and purification. On an analytical scale (40 ml culture), a purification procedure was developed that involves extraction of contaminating proteins by anion exchange chromatography (HGXPRT does not bind under the conditions used), followed by Reactive Red 120 agarose affinity chromatography.
Description

Includes bibliographical references.

Keywords
Chemical Pathology

Reference:

Mbewe, B. 2005. Cloning, expression, purification and drug targeting of Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT). University of Cape Town.

Collections
PhD / Doctoral