Studies on the sucrose utilization system of Clostridium beijerinckii NCIMB 8052

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dc.contributor.advisorReid, Sharon Jen_ZA
dc.contributor.advisorWoods, David Ren_ZA
dc.contributor.authorLeat, Nealen_ZA
dc.date.accessioned2016-07-04T08:42:32Z
dc.date.available2016-07-04T08:42:32Z
dc.date.issued1997en_ZA
dc.descriptionBibliography: pages 117-130.en_ZA
dc.description.abstractSolventogenic clostridial strains were used extensively in the industrial acetone-butanolethanol (ABE) fermentation during the first half of this century. This fermentation was based predominantly on two substrates: corn mash (rich in starch) and molasses (rich in sucrose). Taxonomically distinct strains were used for ABE fermentations based on the two substrates. Although starch . utilization by amylolytic solventogenic clostridial strains had been investigated, sucrose utilization by saccharolytic solventogenic clostridial strains had not been comprehensively studied. This provided the impetus for the present study. The primary aim of the project was to provide a molecular and physiological characterization of the sucrose utilization system of Clostridium beijerinckii NCIMB 8052. The C. beijerinckii sucrose utilization operon, scrARBK, was cloned in three stages. In the first stage a C. beijerinckii genomic library was screened in Escherichia coli for clones able to utilize sucrose. Using this approach, a truncated scr operon was isolated. The remainder of the operon was isolated in the second and third stages by recovering a plasmid integrated into the scrB gene, and inverse-PCR respectively. The four genes of the scrARBK operon were prowsed to encode an Enzyme1IBC1ucrose protein of the PTS (ScrA); a transcriptional repressor of the GalR-Lacl family (ScrR), a sucrose-6-phosphate hydrolase of the glucosyl hydrolase family 32 (ScrB) and an ATP-dependent fructokinase of the ribokinase/pfkB sugar kinase family (ScrK).en_ZA
dc.identifier.apacitationLeat, N. (1997). <i>Studies on the sucrose utilization system of Clostridium beijerinckii NCIMB 8052</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/20193en_ZA
dc.identifier.chicagocitationLeat, Neal. <i>"Studies on the sucrose utilization system of Clostridium beijerinckii NCIMB 8052."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1997. http://hdl.handle.net/11427/20193en_ZA
dc.identifier.citationLeat, N. 1997. Studies on the sucrose utilization system of Clostridium beijerinckii NCIMB 8052. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Leat, Neal AB - Solventogenic clostridial strains were used extensively in the industrial acetone-butanolethanol (ABE) fermentation during the first half of this century. This fermentation was based predominantly on two substrates: corn mash (rich in starch) and molasses (rich in sucrose). Taxonomically distinct strains were used for ABE fermentations based on the two substrates. Although starch . utilization by amylolytic solventogenic clostridial strains had been investigated, sucrose utilization by saccharolytic solventogenic clostridial strains had not been comprehensively studied. This provided the impetus for the present study. The primary aim of the project was to provide a molecular and physiological characterization of the sucrose utilization system of Clostridium beijerinckii NCIMB 8052. The C. beijerinckii sucrose utilization operon, scrARBK, was cloned in three stages. In the first stage a C. beijerinckii genomic library was screened in Escherichia coli for clones able to utilize sucrose. Using this approach, a truncated scr operon was isolated. The remainder of the operon was isolated in the second and third stages by recovering a plasmid integrated into the scrB gene, and inverse-PCR respectively. The four genes of the scrARBK operon were prowsed to encode an Enzyme1IBC1ucrose protein of the PTS (ScrA); a transcriptional repressor of the GalR-Lacl family (ScrR), a sucrose-6-phosphate hydrolase of the glucosyl hydrolase family 32 (ScrB) and an ATP-dependent fructokinase of the ribokinase/pfkB sugar kinase family (ScrK). DA - 1997 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1997 T1 - Studies on the sucrose utilization system of Clostridium beijerinckii NCIMB 8052 TI - Studies on the sucrose utilization system of Clostridium beijerinckii NCIMB 8052 UR - http://hdl.handle.net/11427/20193 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20193
dc.identifier.vancouvercitationLeat N. Studies on the sucrose utilization system of Clostridium beijerinckii NCIMB 8052. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1997 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/20193en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMolecular and Cell Biologyen_ZA
dc.titleStudies on the sucrose utilization system of Clostridium beijerinckii NCIMB 8052en_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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