Cytoplasmic RNA-dependent RNA-synthesis in maturing chicken erythrocytes.

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dc.contributor.advisorFitschen, Wen_ZA
dc.contributor.authorBoyd, Malcolm Charles Daviden_ZA
dc.date.accessioned2015-02-11T14:13:52Z
dc.date.available2015-02-11T14:13:52Z
dc.date.issued1976en_ZA
dc.descriptionIncludes bibliographical references.en_ZA
dc.description.abstractAn RNA-dependent RNA polymerase from ribosomes of maturing chicken erythrocytes was investigated. Concurrent with an increase in globin and RNA synthesis during phenylhydrazine induced anaemia, the total and specific activities of ribosome bound RNA-dependent RNA polymerase increased 40 and 9.4 times respectively, indicating the possible involvement of this enzyme activity in the synthesis or control of synthesis of globin. A partially purified RNA-dependent RNA polymerase was prepared from ribosomes of immature chicken erythrocytes. This preparation was shown to be a predominantly primer dependent enzyme activity, incorporating UTP, CTP and to a lesser extent ATP and GTP into homopoly-ribonucleotide material. The ribosome-bound enzyme preparation demonstrated in addition, the synthesis of heteropolyribonucleotide material. A complementary DNA copy of chicken globin mRNA containing-9S RNP, was prepared. Unlabelled complementary DNA was used to demonstrate that no globin mRNA replicase activity was detectable in the ribosome fraction of immature chicken erythrocytes. The radioactively labelled product obtained after incubation of ribosomes with [³H]-UTP was shown by sucrose density gradient centrifugation to have a sedimentation coefficient of 4S; the ratio of non-terminal to terminal incorporation of UTP was 6.08. The product synthesized after incubation of intact cells in the presence of [³H]-uridine and actinomycin D was shown to be very similar with respect to S-value and the ratio of UMP/uridine incorporated. This similarity suggests the presence of a ribosome bound RNA-dependent RNA polymerase activity in intact cells.en_ZA
dc.identifier.apacitationBoyd, M. C. D. (1976). <i>Cytoplasmic RNA-dependent RNA-synthesis in maturing chicken erythrocytes</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/12447en_ZA
dc.identifier.chicagocitationBoyd, Malcolm Charles David. <i>"Cytoplasmic RNA-dependent RNA-synthesis in maturing chicken erythrocytes."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1976. http://hdl.handle.net/11427/12447en_ZA
dc.identifier.citationBoyd, M. 1976. Cytoplasmic RNA-dependent RNA-synthesis in maturing chicken erythrocytes. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Boyd, Malcolm Charles David AB - An RNA-dependent RNA polymerase from ribosomes of maturing chicken erythrocytes was investigated. Concurrent with an increase in globin and RNA synthesis during phenylhydrazine induced anaemia, the total and specific activities of ribosome bound RNA-dependent RNA polymerase increased 40 and 9.4 times respectively, indicating the possible involvement of this enzyme activity in the synthesis or control of synthesis of globin. A partially purified RNA-dependent RNA polymerase was prepared from ribosomes of immature chicken erythrocytes. This preparation was shown to be a predominantly primer dependent enzyme activity, incorporating UTP, CTP and to a lesser extent ATP and GTP into homopoly-ribonucleotide material. The ribosome-bound enzyme preparation demonstrated in addition, the synthesis of heteropolyribonucleotide material. A complementary DNA copy of chicken globin mRNA containing-9S RNP, was prepared. Unlabelled complementary DNA was used to demonstrate that no globin mRNA replicase activity was detectable in the ribosome fraction of immature chicken erythrocytes. The radioactively labelled product obtained after incubation of ribosomes with [³H]-UTP was shown by sucrose density gradient centrifugation to have a sedimentation coefficient of 4S; the ratio of non-terminal to terminal incorporation of UTP was 6.08. The product synthesized after incubation of intact cells in the presence of [³H]-uridine and actinomycin D was shown to be very similar with respect to S-value and the ratio of UMP/uridine incorporated. This similarity suggests the presence of a ribosome bound RNA-dependent RNA polymerase activity in intact cells. DA - 1976 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1976 T1 - Cytoplasmic RNA-dependent RNA-synthesis in maturing chicken erythrocytes TI - Cytoplasmic RNA-dependent RNA-synthesis in maturing chicken erythrocytes UR - http://hdl.handle.net/11427/12447 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/12447
dc.identifier.vancouvercitationBoyd MCD. Cytoplasmic RNA-dependent RNA-synthesis in maturing chicken erythrocytes. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1976 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/12447en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherBiochemistryen_ZA
dc.titleCytoplasmic RNA-dependent RNA-synthesis in maturing chicken erythrocytes.en_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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