Immunochemical studies with tryptic peptides of tobacco mosaic virus protein

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dc.contributor.advisorVan Regenmortel, M H Ven_ZA
dc.contributor.authorMilton, Raymond Cecil de Lisleen_ZA
dc.date.accessioned2015-03-13T14:06:24Z
dc.date.available2015-03-13T14:06:24Z
dc.date.issued1979en_ZA
dc.descriptionIncludes bibliographical references.en_ZA
dc.description.abstractThe antigenic determinants of the protein subunit of tobacco mosaic virus CTMV) have been studied by inhibition of complement fixation, inhibition of micro-precipitin and direct binding experiments. The viral subunit has been found to possess six antigenic determinants. Two of these, situated in tryptic peptide I (residues 1-41), were found to be a cryptotope (i.e. an antigenic determinant absent from the outer surface of the assembled viral capsid) and a viral antigenic determinant. Tryptic peptides 4 (residues 62-68) and 8(residues 93-112) also contained cryptotopes which were situated in the region of residues 63-65 and 108-112 respectively. Tryptic peptide 12 (residues 142-158) contained both a cryptotope and a neotope (i.e. an antigenic determinant which is only expressed on the outer surface of the viral capsid), which are situated in the C-terminal region of the polypeptide chain of the TMV protein, residue156 being associated with the cryptotope and residue 158 with the neotope. No antigenic activity could be demonstrated in tryptic peptide I I (residues 135-141 ). When the results are analyzed in terms of the three-dimensional structure of the viral subunit, it appears that all the antigenic reactive regions occupy highly accessible locations on the surface of the protein.en_ZA
dc.identifier.apacitationMilton, R. C. d. L. (1979). <i>Immunochemical studies with tryptic peptides of tobacco mosaic virus protein</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/12596en_ZA
dc.identifier.chicagocitationMilton, Raymond Cecil de Lisle. <i>"Immunochemical studies with tryptic peptides of tobacco mosaic virus protein."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1979. http://hdl.handle.net/11427/12596en_ZA
dc.identifier.citationMilton, R. 1979. Immunochemical studies with tryptic peptides of tobacco mosaic virus protein. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Milton, Raymond Cecil de Lisle AB - The antigenic determinants of the protein subunit of tobacco mosaic virus CTMV) have been studied by inhibition of complement fixation, inhibition of micro-precipitin and direct binding experiments. The viral subunit has been found to possess six antigenic determinants. Two of these, situated in tryptic peptide I (residues 1-41), were found to be a cryptotope (i.e. an antigenic determinant absent from the outer surface of the assembled viral capsid) and a viral antigenic determinant. Tryptic peptides 4 (residues 62-68) and 8(residues 93-112) also contained cryptotopes which were situated in the region of residues 63-65 and 108-112 respectively. Tryptic peptide 12 (residues 142-158) contained both a cryptotope and a neotope (i.e. an antigenic determinant which is only expressed on the outer surface of the viral capsid), which are situated in the C-terminal region of the polypeptide chain of the TMV protein, residue156 being associated with the cryptotope and residue 158 with the neotope. No antigenic activity could be demonstrated in tryptic peptide I I (residues 135-141 ). When the results are analyzed in terms of the three-dimensional structure of the viral subunit, it appears that all the antigenic reactive regions occupy highly accessible locations on the surface of the protein. DA - 1979 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1979 T1 - Immunochemical studies with tryptic peptides of tobacco mosaic virus protein TI - Immunochemical studies with tryptic peptides of tobacco mosaic virus protein UR - http://hdl.handle.net/11427/12596 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/12596
dc.identifier.vancouvercitationMilton RCdL. Immunochemical studies with tryptic peptides of tobacco mosaic virus protein. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1979 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/12596en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherBiochemistryen_ZA
dc.titleImmunochemical studies with tryptic peptides of tobacco mosaic virus proteinen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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