A study of the relative dominance of selected anaerobic sulphate-reducing bacteria in a continuous bioreactor revealed by fluorescence in situ hybridisation
| dc.contributor.author | Icgen, B | |
| dc.contributor.author | Moosa, S | |
| dc.contributor.author | Harrison, S T L | |
| dc.date.accessioned | 2016-08-19T11:41:44Z | |
| dc.date.available | 2016-08-19T11:41:44Z | |
| dc.date.issued | 2007 | |
| dc.date.updated | 2016-08-19T08:28:38Z | |
| dc.description.abstract | The diversity and the community structure of sulfate-reducing bacteria (SRB) in an anaerobic continuous bioreactor used for treatment of a sulfate-containing wastewater were investigated by fluorescence in situ hybridization. Hybridization to the 16S rRNA probe EUB338 for the domain Bacteria was performed, followed by a nonsense probe NON338 as a control for nonspecific staining. Sulfate-reducing consortia were identified by using five nominally genus-specific probes (SRB129 for Desulfobacter, SRB221 for Desulfobacterium, SRB228 for Desulfotomaculum, SRB660 for Desulfobulbus, and SRB657 for Desulfonema) and four group-specific probes (SRB385 as a general SRB probe, SRB687 for Desulfovibrioaceae, SRB814 for Desulfococcus group, and SRB804 for Desulfobacteriaceae). The total prokaryotic population was determined by 4′,6-diamidino-2-phenylindole staining. Hybridization analysis using these 16S rRNA-targeted oligonucleotide probes showed that, of those microbial groupings investigated, Desulfonema, Desulfobulbus, spp., and Desulfobacteriaceae group were the main sulfate-reducing bacteria in the bioreactor when operated at steady state at 35°C, pH 7.8, and a 2.5-day residence time with feed stream containing 2.5 kg m−3 sulfate as terminal electron acceptor and 2.3 kg m−3 acetate as carbon source and electron donor. | en_ZA |
| dc.identifier | http://dx.doi.org/10.1007/s00248-006-9009-0 | |
| dc.identifier.apacitation | Icgen, B., Moosa, S., & Harrison, S. T. L. (2007). A study of the relative dominance of selected anaerobic sulphate-reducing bacteria in a continuous bioreactor revealed by fluorescence in situ hybridisation. <i>Microbial Ecology</i>, http://hdl.handle.net/11427/21361 | en_ZA |
| dc.identifier.chicagocitation | Icgen, B, S Moosa, and S T L Harrison "A study of the relative dominance of selected anaerobic sulphate-reducing bacteria in a continuous bioreactor revealed by fluorescence in situ hybridisation." <i>Microbial Ecology</i> (2007) http://hdl.handle.net/11427/21361 | en_ZA |
| dc.identifier.citation | Icgen, B., Moosa, S., & Harrison, S. T. L. (2007). A study of the relative dominance of selected anaerobic sulfate-reducing bacteria in a continuous bioreactor by fluorescence in situ hybridization. Microbial ecology, 53(1), 43-52. | en_ZA |
| dc.identifier.issn | 0095-3628 | en_ZA |
| dc.identifier.ris | TY - Journal Article AU - Icgen, B AU - Moosa, S AU - Harrison, S T L AB - The diversity and the community structure of sulfate-reducing bacteria (SRB) in an anaerobic continuous bioreactor used for treatment of a sulfate-containing wastewater were investigated by fluorescence in situ hybridization. Hybridization to the 16S rRNA probe EUB338 for the domain Bacteria was performed, followed by a nonsense probe NON338 as a control for nonspecific staining. Sulfate-reducing consortia were identified by using five nominally genus-specific probes (SRB129 for Desulfobacter, SRB221 for Desulfobacterium, SRB228 for Desulfotomaculum, SRB660 for Desulfobulbus, and SRB657 for Desulfonema) and four group-specific probes (SRB385 as a general SRB probe, SRB687 for Desulfovibrioaceae, SRB814 for Desulfococcus group, and SRB804 for Desulfobacteriaceae). The total prokaryotic population was determined by 4′,6-diamidino-2-phenylindole staining. Hybridization analysis using these 16S rRNA-targeted oligonucleotide probes showed that, of those microbial groupings investigated, Desulfonema, Desulfobulbus, spp., and Desulfobacteriaceae group were the main sulfate-reducing bacteria in the bioreactor when operated at steady state at 35°C, pH 7.8, and a 2.5-day residence time with feed stream containing 2.5 kg m−3 sulfate as terminal electron acceptor and 2.3 kg m−3 acetate as carbon source and electron donor. DA - 2007 DB - OpenUCT DP - University of Cape Town J1 - Microbial Ecology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2007 SM - 0095-3628 T1 - A study of the relative dominance of selected anaerobic sulphate-reducing bacteria in a continuous bioreactor revealed by fluorescence in situ hybridisation TI - A study of the relative dominance of selected anaerobic sulphate-reducing bacteria in a continuous bioreactor revealed by fluorescence in situ hybridisation UR - http://hdl.handle.net/11427/21361 ER - | en_ZA |
| dc.identifier.uri | http://hdl.handle.net/11427/21361 | |
| dc.identifier.vancouvercitation | Icgen B, Moosa S, Harrison STL. A study of the relative dominance of selected anaerobic sulphate-reducing bacteria in a continuous bioreactor revealed by fluorescence in situ hybridisation. Microbial Ecology. 2007; http://hdl.handle.net/11427/21361. | en_ZA |
| dc.language | eng | en_ZA |
| dc.publisher | Springer | en_ZA |
| dc.publisher.institution | University of Cape Town | |
| dc.source | Microbial Ecology | en_ZA |
| dc.source.uri | http://link.springer.com/journal/248 | |
| dc.title | A study of the relative dominance of selected anaerobic sulphate-reducing bacteria in a continuous bioreactor revealed by fluorescence in situ hybridisation | en_ZA |
| dc.type | Journal Article | en_ZA |
| uct.type.filetype | Text | |
| uct.type.filetype | Image | |
| uct.type.publication | Research | en_ZA |
| uct.type.resource | Article | en_ZA |