A study of the relative dominance of selected anaerobic sulphate-reducing bacteria in a continuous bioreactor revealed by fluorescence in situ hybridisation
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2007
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Microbial Ecology
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Springer
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University of Cape Town
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Abstract
The diversity and the community structure of sulfate-reducing bacteria (SRB) in an anaerobic continuous bioreactor used for treatment of a sulfate-containing wastewater were investigated by fluorescence in situ hybridization. Hybridization to the 16S rRNA probe EUB338 for the domain Bacteria was performed, followed by a nonsense probe NON338 as a control for nonspecific staining. Sulfate-reducing consortia were identified by using five nominally genus-specific probes (SRB129 for Desulfobacter, SRB221 for Desulfobacterium, SRB228 for Desulfotomaculum, SRB660 for Desulfobulbus, and SRB657 for Desulfonema) and four group-specific probes (SRB385 as a general SRB probe, SRB687 for Desulfovibrioaceae, SRB814 for Desulfococcus group, and SRB804 for Desulfobacteriaceae). The total prokaryotic population was determined by 4′,6-diamidino-2-phenylindole staining. Hybridization analysis using these 16S rRNA-targeted oligonucleotide probes showed that, of those microbial groupings investigated, Desulfonema, Desulfobulbus, spp., and Desulfobacteriaceae group were the main sulfate-reducing bacteria in the bioreactor when operated at steady state at 35°C, pH 7.8, and a 2.5-day residence time with feed stream containing 2.5 kg m−3 sulfate as terminal electron acceptor and 2.3 kg m−3 acetate as carbon source and electron donor.
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Icgen, B., Moosa, S., & Harrison, S. T. L. (2007). A study of the relative dominance of selected anaerobic sulfate-reducing bacteria in a continuous bioreactor by fluorescence in situ hybridization. Microbial ecology, 53(1), 43-52.