Post-translational processing of the low density lipoprotein receptor

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dc.contributor.advisorVan der Westhuyzen, Deneys Ren_ZA
dc.contributor.authorOzinsky, Adrianen_ZA
dc.date.accessioned2017-12-14T14:20:10Z
dc.date.available2017-12-14T14:20:10Z
dc.date.issued1996en_ZA
dc.description.abstractThe low density lipoprotein (LDL) receptor is a transmembrane glycoprotein that mediates the uptake of plasma LDL and thereby provides cholesterol to cells. During its synthesis in the endoplasmic reticulum, the LDL receptor folds and forms disulfide bonds in multiple cysteine-rich repeats. N- and 0-linked oligosaccharide chains are added in the endoplasmic reticulum and processed during passage through the Golgi apparatus, en route to the cell surface. The aim of this thesis was to study the influence of post-translational events on the synthesis of the LDL receptor. Experiments addressed: 1) the necessity of the compartmental organisation of the secretory pathway for the glycosylation of the LDL receptor; 2) the requirements for the formation of disulfide bonds; 3) the role for the chaperone, calnexin, in the folding of the LDL receptor; and 4) the manner in which folding was disrupted by mutations. Experiments were performed in cultured cells that were incubated with [³⁵S]methionine. Biosynthetically-labelled LDL receptor was immunoprecipitated and was analysed by SOS polyacrylamide gel electrophoresis.en_ZA
dc.identifier.apacitationOzinsky, A. (1996). <i>Post-translational processing of the low density lipoprotein receptor</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology. Retrieved from http://hdl.handle.net/11427/26672en_ZA
dc.identifier.chicagocitationOzinsky, Adrian. <i>"Post-translational processing of the low density lipoprotein receptor."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology, 1996. http://hdl.handle.net/11427/26672en_ZA
dc.identifier.citationOzinsky, A. 1996. Post-translational processing of the low density lipoprotein receptor. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Ozinsky, Adrian AB - The low density lipoprotein (LDL) receptor is a transmembrane glycoprotein that mediates the uptake of plasma LDL and thereby provides cholesterol to cells. During its synthesis in the endoplasmic reticulum, the LDL receptor folds and forms disulfide bonds in multiple cysteine-rich repeats. N- and 0-linked oligosaccharide chains are added in the endoplasmic reticulum and processed during passage through the Golgi apparatus, en route to the cell surface. The aim of this thesis was to study the influence of post-translational events on the synthesis of the LDL receptor. Experiments addressed: 1) the necessity of the compartmental organisation of the secretory pathway for the glycosylation of the LDL receptor; 2) the requirements for the formation of disulfide bonds; 3) the role for the chaperone, calnexin, in the folding of the LDL receptor; and 4) the manner in which folding was disrupted by mutations. Experiments were performed in cultured cells that were incubated with [³⁵S]methionine. Biosynthetically-labelled LDL receptor was immunoprecipitated and was analysed by SOS polyacrylamide gel electrophoresis. DA - 1996 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1996 T1 - Post-translational processing of the low density lipoprotein receptor TI - Post-translational processing of the low density lipoprotein receptor UR - http://hdl.handle.net/11427/26672 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/26672
dc.identifier.vancouvercitationOzinsky A. Post-translational processing of the low density lipoprotein receptor. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology, 1996 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/26672en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Medical Biochemistry and Structural Biology
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherReceptors, LDL - biosynthesisen_ZA
dc.subject.otherReceptors, LDL - chemistryen_ZA
dc.subject.otherMedical Biochemistryen_ZA
dc.titlePost-translational processing of the low density lipoprotein receptoren_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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