In silico investigation of the mechanism of ricin-catalysed depurination reaction and design of novel ricin inhibitors

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dc.contributor.advisorNaidoo, Kevin Jen_ZA
dc.contributor.authorJayakody, Ranga Sen_ZA
dc.date.accessioned2015-01-10T13:42:13Z
dc.date.available2015-01-10T13:42:13Z
dc.date.issued2012en_ZA
dc.description.abstractRicin is a dimeric enzyme found in the castor bean plant. It is extremely toxic with a fatal dose for humans ranging from 0.1-1.0 ug/kg. This has lead to its use as a biological weapon. Cell death is caused when ricin ceases the protein synthesis by removing a specific adenine (A-4324) of the GAGA tetra loop of 28S ribosomal RNA. Despite this destructive feature, ricin has been touted as a potential therapeutic agent where applications such as immunotoxins to treat cancer, AIDS and other diseases are actively being pursued. However, the prime challenge in such applications is the non specific cytotoxicity of ricin, which cannot currently be treated due to the absence of an effective antidote. The primary objective of this thesis is to describe the catalytic mechanism of ricin using computational reaction dynamics. For an accurate simulation of the ricin-catalysed reaction, a reasonable model of the target natural substrate is required.en_ZA
dc.identifier.apacitationJayakody, R. S. (2012). <i>In silico investigation of the mechanism of ricin-catalysed depurination reaction and design of novel ricin inhibitors</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Chemistry. Retrieved from http://hdl.handle.net/11427/12021en_ZA
dc.identifier.chicagocitationJayakody, Ranga S. <i>"In silico investigation of the mechanism of ricin-catalysed depurination reaction and design of novel ricin inhibitors."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Chemistry, 2012. http://hdl.handle.net/11427/12021en_ZA
dc.identifier.citationJayakody, R. 2012. In silico investigation of the mechanism of ricin-catalysed depurination reaction and design of novel ricin inhibitors. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Jayakody, Ranga S AB - Ricin is a dimeric enzyme found in the castor bean plant. It is extremely toxic with a fatal dose for humans ranging from 0.1-1.0 ug/kg. This has lead to its use as a biological weapon. Cell death is caused when ricin ceases the protein synthesis by removing a specific adenine (A-4324) of the GAGA tetra loop of 28S ribosomal RNA. Despite this destructive feature, ricin has been touted as a potential therapeutic agent where applications such as immunotoxins to treat cancer, AIDS and other diseases are actively being pursued. However, the prime challenge in such applications is the non specific cytotoxicity of ricin, which cannot currently be treated due to the absence of an effective antidote. The primary objective of this thesis is to describe the catalytic mechanism of ricin using computational reaction dynamics. For an accurate simulation of the ricin-catalysed reaction, a reasonable model of the target natural substrate is required. DA - 2012 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2012 T1 - In silico investigation of the mechanism of ricin-catalysed depurination reaction and design of novel ricin inhibitors TI - In silico investigation of the mechanism of ricin-catalysed depurination reaction and design of novel ricin inhibitors UR - http://hdl.handle.net/11427/12021 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/12021
dc.identifier.vancouvercitationJayakody RS. In silico investigation of the mechanism of ricin-catalysed depurination reaction and design of novel ricin inhibitors. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Chemistry, 2012 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/12021en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Chemistryen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherChemistryen_ZA
dc.titleIn silico investigation of the mechanism of ricin-catalysed depurination reaction and design of novel ricin inhibitorsen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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