The importance of N-linked glycosylation on the N-domain of angiotensin-I converting enzyme

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dc.contributor.advisorSturrock, Edward Den_ZA
dc.contributor.authorAnthony, Colin Scotten_ZA
dc.date.accessioned2014-12-26T06:18:48Z
dc.date.available2014-12-26T06:18:48Z
dc.date.issued2011en_ZA
dc.description.abstractAngiotensin-I converting enzyme (ACE) is an important drug target in the treatment of heart disease due to its role in the regulation of blood pressure. ACE contains two domains, the N- and C-domains, both of which are catalytically active and heavily glycosylated. Glycosylation is one of the most important forms of post-translational modification, having a wide range of functions including protein folding, modulation of the immune response, and providing targeting signals. Glycosylation is required for the expression of active ACE and structural studies of ACE have been fraught with severe difficulties because of surface N-glycosylation of the protein. This problem has been addressed to a large extent with respect to the C-domain, where the role of glycosylation has been extensively characterised and a minimally glycosylated form was able to crystallise reproducibly. As yet, little is known about the degree and importance of N-linked glycosylation on the N-domain. The generation of minimally glycosylated N-domain, however, requires a greater understanding of the relative importance of the individual N-linked glycosylation sites.en_ZA
dc.identifier.apacitationAnthony, C. S. (2011). <i>The importance of N-linked glycosylation on the N-domain of angiotensin-I converting enzyme</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry. Retrieved from http://hdl.handle.net/11427/10051en_ZA
dc.identifier.chicagocitationAnthony, Colin Scott. <i>"The importance of N-linked glycosylation on the N-domain of angiotensin-I converting enzyme."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry, 2011. http://hdl.handle.net/11427/10051en_ZA
dc.identifier.citationAnthony, C. 2011. The importance of N-linked glycosylation on the N-domain of angiotensin-I converting enzyme. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Anthony, Colin Scott AB - Angiotensin-I converting enzyme (ACE) is an important drug target in the treatment of heart disease due to its role in the regulation of blood pressure. ACE contains two domains, the N- and C-domains, both of which are catalytically active and heavily glycosylated. Glycosylation is one of the most important forms of post-translational modification, having a wide range of functions including protein folding, modulation of the immune response, and providing targeting signals. Glycosylation is required for the expression of active ACE and structural studies of ACE have been fraught with severe difficulties because of surface N-glycosylation of the protein. This problem has been addressed to a large extent with respect to the C-domain, where the role of glycosylation has been extensively characterised and a minimally glycosylated form was able to crystallise reproducibly. As yet, little is known about the degree and importance of N-linked glycosylation on the N-domain. The generation of minimally glycosylated N-domain, however, requires a greater understanding of the relative importance of the individual N-linked glycosylation sites. DA - 2011 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2011 T1 - The importance of N-linked glycosylation on the N-domain of angiotensin-I converting enzyme TI - The importance of N-linked glycosylation on the N-domain of angiotensin-I converting enzyme UR - http://hdl.handle.net/11427/10051 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/10051
dc.identifier.vancouvercitationAnthony CS. The importance of N-linked glycosylation on the N-domain of angiotensin-I converting enzyme. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry, 2011 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/10051en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Medical Biochemistryen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMedical Biochemistryen_ZA
dc.titleThe importance of N-linked glycosylation on the N-domain of angiotensin-I converting enzymeen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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