Genome-wide survey and analysis of allele-specific mRNA splicing in human and mouse

dc.contributor.advisorSeoighe, Cathalen_ZA
dc.contributor.authorNembaware, Victoria Preciousen_ZA
dc.date.accessioned2014-07-30T17:40:29Z
dc.date.available2014-07-30T17:40:29Z
dc.date.issued2008en_ZA
dc.descriptionIncludes abstract.
dc.descriptionIncludes bibliographical references (leaves 125-145).
dc.description.abstractThis dissertation aims to examine allele-specific splicing in human and mouse using publicly available datasets. Such datasets, which have been generated from multiple tissue sources and from individuals of diverse backgrounds, are rich and cheap reservoirs of transcript isoforms resulting from alternative splicing as well as isoforms resulting from mutations or polymorphisms (allele-specific isoforms). Published tools were used to analyse microarray and genomic data. However, for the assessment of allele-specific splicing using publicly available high-throughput transcript sequences, we present two novel methods: a heuristic method for quantifying the prevalence of allele-specific splicing and a more sophisticated maximum likelihood method for the detection of individual examples of allele-specific splicing. These methods make use of transcripts that can be mapped to both polymorphisms and computationally predicted mRNA isoforms. Inference of polymorphic alleles from transcripts is laborious hence a pre-computed database was created for the human data and made publicly available for use by the wider research community.en_ZA
dc.identifier.apacitationNembaware, V. P. (2008). <i>Genome-wide survey and analysis of allele-specific mRNA splicing in human and mouse</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/4310en_ZA
dc.identifier.chicagocitationNembaware, Victoria Precious. <i>"Genome-wide survey and analysis of allele-specific mRNA splicing in human and mouse."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2008. http://hdl.handle.net/11427/4310en_ZA
dc.identifier.citationNembaware, V. 2008. Genome-wide survey and analysis of allele-specific mRNA splicing in human and mouse. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Nembaware, Victoria Precious AB - This dissertation aims to examine allele-specific splicing in human and mouse using publicly available datasets. Such datasets, which have been generated from multiple tissue sources and from individuals of diverse backgrounds, are rich and cheap reservoirs of transcript isoforms resulting from alternative splicing as well as isoforms resulting from mutations or polymorphisms (allele-specific isoforms). Published tools were used to analyse microarray and genomic data. However, for the assessment of allele-specific splicing using publicly available high-throughput transcript sequences, we present two novel methods: a heuristic method for quantifying the prevalence of allele-specific splicing and a more sophisticated maximum likelihood method for the detection of individual examples of allele-specific splicing. These methods make use of transcripts that can be mapped to both polymorphisms and computationally predicted mRNA isoforms. Inference of polymorphic alleles from transcripts is laborious hence a pre-computed database was created for the human data and made publicly available for use by the wider research community. DA - 2008 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2008 T1 - Genome-wide survey and analysis of allele-specific mRNA splicing in human and mouse TI - Genome-wide survey and analysis of allele-specific mRNA splicing in human and mouse UR - http://hdl.handle.net/11427/4310 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/4310
dc.identifier.vancouvercitationNembaware VP. Genome-wide survey and analysis of allele-specific mRNA splicing in human and mouse. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2008 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/4310en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleGenome-wide survey and analysis of allele-specific mRNA splicing in human and mouseen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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