Expression of HPV-16 L2 in plants

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dc.contributor.advisorRybicki, Eden_ZA
dc.contributor.advisorBecker, Ingaen_ZA
dc.contributor.advisorMaclean, Jamesen_ZA
dc.contributor.authorPereira, Romanen_ZA
dc.date.accessioned2014-07-30T17:41:28Z
dc.date.available2014-07-30T17:41:28Z
dc.date.issued2008en_ZA
dc.descriptionIncludes bibliographical references (leaves 77-84).
dc.description.abstractThis study demonstrates high level expression of L2, 25 mg.kg⁻¹of leaf material, is achievable. Interestingly, expression was best when coded for by a mammalian codon-optimized form of the L2 gene as opposed to the wildtype or plant codon-optimized (plantized) genes. Moreover, real time PCR revealed limited levels of transcript when coded for by the plantized gene in comparison to the other genes. A set of vectors which target the protein to the cytoplasm, the endoplasmic reticulum (ER), chloroplast or apoplastic space were Expression of HPV -16 L2 in Plants used and it was found that targeting of the protein had no effect on its expression levels.en_ZA
dc.identifier.apacitationPereira, R. (2008). <i>Expression of HPV-16 L2 in plants</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/4317en_ZA
dc.identifier.chicagocitationPereira, Roman. <i>"Expression of HPV-16 L2 in plants."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2008. http://hdl.handle.net/11427/4317en_ZA
dc.identifier.citationPereira, R. 2008. Expression of HPV-16 L2 in plants. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Pereira, Roman AB - This study demonstrates high level expression of L2, 25 mg.kg⁻¹of leaf material, is achievable. Interestingly, expression was best when coded for by a mammalian codon-optimized form of the L2 gene as opposed to the wildtype or plant codon-optimized (plantized) genes. Moreover, real time PCR revealed limited levels of transcript when coded for by the plantized gene in comparison to the other genes. A set of vectors which target the protein to the cytoplasm, the endoplasmic reticulum (ER), chloroplast or apoplastic space were Expression of HPV -16 L2 in Plants used and it was found that targeting of the protein had no effect on its expression levels. DA - 2008 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2008 T1 - Expression of HPV-16 L2 in plants TI - Expression of HPV-16 L2 in plants UR - http://hdl.handle.net/11427/4317 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/4317
dc.identifier.vancouvercitationPereira R. Expression of HPV-16 L2 in plants. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2008 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/4317en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleExpression of HPV-16 L2 in plantsen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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