Expression of HPV-16 L2 in plants
Master Thesis
2008
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University of Cape Town
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Abstract
This study demonstrates high level expression of L2, 25 mg.kg⁻¹of leaf material, is achievable. Interestingly, expression was best when coded for by a mammalian codon-optimized form of the L2 gene as opposed to the wildtype or plant codon-optimized (plantized) genes. Moreover, real time PCR revealed limited levels of transcript when coded for by the plantized gene in comparison to the other genes. A set of vectors which target the protein to the cytoplasm, the endoplasmic reticulum (ER), chloroplast or apoplastic space were Expression of HPV -16 L2 in Plants used and it was found that targeting of the protein had no effect on its expression levels.
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Includes bibliographical references (leaves 77-84).
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Pereira, R. 2008. Expression of HPV-16 L2 in plants. University of Cape Town.