Development of a transformation protocol and cell culture system for the commercially important species of red macroalga, Gracilaria gracilis

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dc.contributor.advisorCoyne, Vernonen_ZA
dc.contributor.advisorMeyers, Annen_ZA
dc.contributor.authorHuddy, Suzanne Margareten_ZA
dc.date.accessioned2014-07-30T17:38:31Z
dc.date.available2014-07-30T17:38:31Z
dc.date.issued2011en_ZA
dc.descriptionIncludes abstract.
dc.descriptionIncludes bibliographical references.
dc.description.abstractA better understanding of how this commercially important seaweed responds at a genetic level to stresses faced in the aquaculture environment would not only be advantageous to the South African industry, but this knowledge is essential for selecting and/or engineering macroalgal strains that are either more tolerant or resistant to these stresses. This requires in vivo analysis of G. gracilis gene function and regulation in order to introduce new or improved genes into G. gracilis, and for this to be possible, a protocol for transformation of recombinant DNA into G. gracilis is required. In this study a transformation and tissue culture system for G. gracilis was developed. These tools provide the necessary groundwork for future genetic manipulation studies that are essential for improving our understanding of the role that various genes play in stress response and tolerance in G. gracilis.en_ZA
dc.identifier.apacitationHuddy, S. M. (2011). <i>Development of a transformation protocol and cell culture system for the commercially important species of red macroalga, Gracilaria gracilis</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/4274en_ZA
dc.identifier.chicagocitationHuddy, Suzanne Margaret. <i>"Development of a transformation protocol and cell culture system for the commercially important species of red macroalga, Gracilaria gracilis."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2011. http://hdl.handle.net/11427/4274en_ZA
dc.identifier.citationHuddy, S. 2011. Development of a transformation protocol and cell culture system for the commercially important species of red macroalga, Gracilaria gracilis. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Huddy, Suzanne Margaret AB - A better understanding of how this commercially important seaweed responds at a genetic level to stresses faced in the aquaculture environment would not only be advantageous to the South African industry, but this knowledge is essential for selecting and/or engineering macroalgal strains that are either more tolerant or resistant to these stresses. This requires in vivo analysis of G. gracilis gene function and regulation in order to introduce new or improved genes into G. gracilis, and for this to be possible, a protocol for transformation of recombinant DNA into G. gracilis is required. In this study a transformation and tissue culture system for G. gracilis was developed. These tools provide the necessary groundwork for future genetic manipulation studies that are essential for improving our understanding of the role that various genes play in stress response and tolerance in G. gracilis. DA - 2011 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2011 T1 - Development of a transformation protocol and cell culture system for the commercially important species of red macroalga, Gracilaria gracilis TI - Development of a transformation protocol and cell culture system for the commercially important species of red macroalga, Gracilaria gracilis UR - http://hdl.handle.net/11427/4274 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/4274
dc.identifier.vancouvercitationHuddy SM. Development of a transformation protocol and cell culture system for the commercially important species of red macroalga, Gracilaria gracilis. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2011 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/4274en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleDevelopment of a transformation protocol and cell culture system for the commercially important species of red macroalga, Gracilaria gracilisen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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