Investigation of carbon catabolite repression in Clostridium beijerinckii NCIMB 8052
Doctoral Thesis
2001
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University of Cape Town
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Abstract
The substrate basis for the industrial acetone-butanol-ethanol (ABE) fermentations, has been agricultural products rich in starch or sucrose, and employed taxonomically distinct amylolytic and saccharolytic solventogenic clostridial strains respectively. There is evidence to suggest that the utilization of these substrates is subject to carbon catabolite repression. In Gram-positive bacteria, carbon catabolite repression is controlled by a global regulatory mechanism, central to which is an imperfect palindromic sequence, the cre element, which is recognized by a protein of the GalR-LacI family, the CcpA protein. A ccpA homologue, regA, has been previously identified in C. acetobutylicum NCP262 and successfully complemented a B. subtilis ccpA mutant strain. The sucrose operon from C. beijerinckii NCIMB 8052, scrARBK, has been characterised at the physiological and genetic levels with the ScrR repressor found to negatively auto-regulate the operon.
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Rafudeen, M. 2001. Investigation of carbon catabolite repression in Clostridium beijerinckii NCIMB 8052. University of Cape Town.