The characterisation of Ornithogalum mosaic virus

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dc.contributor.advisorVon Wechmar, M Barbaraen_ZA
dc.contributor.authorBurger, Johan Theodorusen_ZA
dc.date.accessioned2016-09-20T12:30:58Z
dc.date.available2016-09-20T12:30:58Z
dc.date.issued1991en_ZA
dc.descriptionBibliography: pages 155-179.en_ZA
dc.description.abstractOrnithogalum mosaic virus (OMV) is the most serious pathogen of commercially grown Ornithogalum and Lachenalia species in South Africa. Although omithogalum mosaic disease was first reported as early as 1940, attempts to purify or characterise the virus(es) were not successful. The extremely mucilaginous nature of omithogalum and lachenalia plant extracts severely hampered virus purification from these hosts. No alternative propagation host for OMV is known: a virus purification protocol for systemically infected ornithogalum and lachenalia was therefore developed. This method eliminated the mucilage in leaf extracts by hemicellulase digestion. Physicochemical characterisation of purified particles suggested that a single virus was present: it had elongated, filamentous particles with a modal length in the range 720- 760 nm; a single major coat protein of Mᵣ30 000, and a single genomic ssRNA of Mᵣ2.90 x 10⁶ daltons. Oligo(dT)-cellulose chromatography confirmed that the genomic RNA was polyadenylated.en_ZA
dc.identifier.apacitationBurger, J. T. (1991). <i>The characterisation of Ornithogalum mosaic virus</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/21824en_ZA
dc.identifier.chicagocitationBurger, Johan Theodorus. <i>"The characterisation of Ornithogalum mosaic virus."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1991. http://hdl.handle.net/11427/21824en_ZA
dc.identifier.citationBurger, J. 1991. The characterisation of Ornithogalum mosaic virus. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Burger, Johan Theodorus AB - Ornithogalum mosaic virus (OMV) is the most serious pathogen of commercially grown Ornithogalum and Lachenalia species in South Africa. Although omithogalum mosaic disease was first reported as early as 1940, attempts to purify or characterise the virus(es) were not successful. The extremely mucilaginous nature of omithogalum and lachenalia plant extracts severely hampered virus purification from these hosts. No alternative propagation host for OMV is known: a virus purification protocol for systemically infected ornithogalum and lachenalia was therefore developed. This method eliminated the mucilage in leaf extracts by hemicellulase digestion. Physicochemical characterisation of purified particles suggested that a single virus was present: it had elongated, filamentous particles with a modal length in the range 720- 760 nm; a single major coat protein of Mᵣ30 000, and a single genomic ssRNA of Mᵣ2.90 x 10⁶ daltons. Oligo(dT)-cellulose chromatography confirmed that the genomic RNA was polyadenylated. DA - 1991 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1991 T1 - The characterisation of Ornithogalum mosaic virus TI - The characterisation of Ornithogalum mosaic virus UR - http://hdl.handle.net/11427/21824 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21824
dc.identifier.vancouvercitationBurger JT. The characterisation of Ornithogalum mosaic virus. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1991 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/21824en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMosaic diseasesen_ZA
dc.subject.otherMolecular and Cell Biologyen_ZA
dc.titleThe characterisation of Ornithogalum mosaic virusen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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