Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262

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dc.contributor.advisorReid, Sharon Jen_ZA
dc.contributor.authorCollett, Helen Jeanneen_ZA
dc.date.accessioned2014-11-05T17:31:24Z
dc.date.available2014-11-05T17:31:24Z
dc.date.issued1996en_ZA
dc.descriptionBibliography: leaves [168]-207.en_ZA
dc.description.abstractMetronidazole is a pro-drug which must be reduced to elicit a bactericidal effect. In the clostridia, some of the electron transport proteins that provide the source of electrons for the reductive activation of metronidazole play a key role in electron distribution, which in turn regulates the direction of carbon flow in the cell. The aim of this research project was to isolate electron transport gene(s) from the solvent-producing Clostridium acetobutylicum strain P262, using transposon-induced metronidazole resistance as a selection system. In the process, the feasibility of transposon mutagenesis in this strain, which lacks conventional systems for DNA delivery, was assessed, and the nature of metronidazole susceptibility in the C. acetobutylicum wild type was investigated. The metronidazole resistant transconjugant of interest, referred to as mutant 3R, was shown to harbour a single insertion of the Tn925: :Tn917 transposon cointegrate within a structural gene, designated sum (susceptibility to metronidazole).en_ZA
dc.identifier.apacitationCollett, H. J. (1996). <i>Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/9243en_ZA
dc.identifier.chicagocitationCollett, Helen Jeanne. <i>"Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1996. http://hdl.handle.net/11427/9243en_ZA
dc.identifier.citationCollett, H. 1996. Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Collett, Helen Jeanne AB - Metronidazole is a pro-drug which must be reduced to elicit a bactericidal effect. In the clostridia, some of the electron transport proteins that provide the source of electrons for the reductive activation of metronidazole play a key role in electron distribution, which in turn regulates the direction of carbon flow in the cell. The aim of this research project was to isolate electron transport gene(s) from the solvent-producing Clostridium acetobutylicum strain P262, using transposon-induced metronidazole resistance as a selection system. In the process, the feasibility of transposon mutagenesis in this strain, which lacks conventional systems for DNA delivery, was assessed, and the nature of metronidazole susceptibility in the C. acetobutylicum wild type was investigated. The metronidazole resistant transconjugant of interest, referred to as mutant 3R, was shown to harbour a single insertion of the Tn925: :Tn917 transposon cointegrate within a structural gene, designated sum (susceptibility to metronidazole). DA - 1996 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1996 T1 - Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262 TI - Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262 UR - http://hdl.handle.net/11427/9243 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/9243
dc.identifier.vancouvercitationCollett HJ. Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1996 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/9243en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMicrobiologyen_ZA
dc.titleCharacterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262en_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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