Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis

Simple item page
dc.contributor.advisorDheda, Keertanen_ZA
dc.contributor.authorFanampe, Boitumelo Louisaen_ZA
dc.date.accessioned2016-07-08T10:43:27Z
dc.date.available2016-07-08T10:43:27Z
dc.date.issued2015en_ZA
dc.description.abstractTuberculosis (TB) is a major global health problem. About 15-20% of the global population who are HIV negative have extra-pulmonary TB (EPTB) such as pleural TB. This increases to 50-70% in HIV positive people. The diagnosis of EPTB is challenging because of the low bacillary burden. Interferon gamma (IFN-γ) has been identified as a promising biomarker for the diagnosis of EPTB. The development of rapid and accurate point-of-care (POC) diagnostic technologies becomes crucial in controlling EPTB. Aptamers referred to as "synthetic antibodies" have been recently explored as a replacement for antibodies in diagnostic platforms. These single-stranded nucleic acid molecules have high affinity and specificity comparable, and in some instances even superior, to those of antibodies; in addition to their relatively low cost and simple method of production they have the potential to reduce assay turnaround time. The aim of this thesis was to develop aptamers to IFN-γ, a biomarker specific for EPTB, thus facilitating the development of aptamer-based POC tests for the diagnosis of EPTB.en_ZA
dc.identifier.apacitationFanampe, B. L. (2015). <i>Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Department of Medicine. Retrieved from http://hdl.handle.net/11427/20263en_ZA
dc.identifier.chicagocitationFanampe, Boitumelo Louisa. <i>"Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Department of Medicine, 2015. http://hdl.handle.net/11427/20263en_ZA
dc.identifier.citationFanampe, B. 2015. Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Fanampe, Boitumelo Louisa AB - Tuberculosis (TB) is a major global health problem. About 15-20% of the global population who are HIV negative have extra-pulmonary TB (EPTB) such as pleural TB. This increases to 50-70% in HIV positive people. The diagnosis of EPTB is challenging because of the low bacillary burden. Interferon gamma (IFN-γ) has been identified as a promising biomarker for the diagnosis of EPTB. The development of rapid and accurate point-of-care (POC) diagnostic technologies becomes crucial in controlling EPTB. Aptamers referred to as "synthetic antibodies" have been recently explored as a replacement for antibodies in diagnostic platforms. These single-stranded nucleic acid molecules have high affinity and specificity comparable, and in some instances even superior, to those of antibodies; in addition to their relatively low cost and simple method of production they have the potential to reduce assay turnaround time. The aim of this thesis was to develop aptamers to IFN-γ, a biomarker specific for EPTB, thus facilitating the development of aptamer-based POC tests for the diagnosis of EPTB. DA - 2015 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2015 T1 - Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis TI - Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis UR - http://hdl.handle.net/11427/20263 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20263
dc.identifier.vancouvercitationFanampe BL. Interferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosis. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Department of Medicine, 2015 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/20263en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Medicineen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMedicineen_ZA
dc.titleInterferon-γ aptamers for the diagnosis of extra-pulmonary tuberculosisen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
thesis_hsf_2015_fanampe_boitumelo_louisa (1).pdf
Size:
3.47 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
PhD / Doctoral