A proteomic investigation of the rhizomes of the resurrection fern Mohria caffrorum L. (Desv.) in response to desiccation

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dc.contributor.advisorFarrant, Jill Men_ZA
dc.contributor.advisorRafudeen, Mohamed Sen_ZA
dc.contributor.authorShoko, Rymanen_ZA
dc.date.accessioned2016-02-05T07:19:40Z
dc.date.available2016-02-05T07:19:40Z
dc.date.issued2015en_ZA
dc.descriptionIncludes bibliographical referencesen_ZA
dc.description.abstractAs there is limited information on the mechanisms of vegetative desiccation-tolerance in resurrection plant rhizomes, this work was undertaken to study the mechanisms of desiccation-tolerance in Mohria caffrorum rhizomes. Fronds of this plant have been previously characterized as being desiccation-tolerant in summer and desiccation-sensitive in winter. Since fern rhizomes are perennial organs, it was of interest to establish whether these organs are also perennially desiccation-tolerant and, whether or not the rhizomes regulate desiccation-tolerance in the fronds. Ultra-structural evidence using transmission electron microscopy and viability studies using electrolyte leakage analysis showed that the rhizomes were desiccation-tolerant throughout the seasons. Quantitative proteomics analysis using isobaric tags for relative and absolute quantification was employed to investigate molecular mechanisms of desiccation-tolerance in the rhizomes of this plant. Using a custom fern rhizome specific peptide sequence database, 236 proteins were identified. Of these, 16 proteins increased in abundance while 14 declined, in the summer collected rhizomes. On the other hand, 16 proteins increased in abundance and 20 declined in the winter form. Western blot analysis confirmed the expression trends of heat shock protein 70-2 and superoxide dismutase-[Cu-Zn], which were among the differentially expressed proteins. Bioinformatics analysis of the differentially expressed proteins was carried out using network enrichment tools, to identify key molecular processes and pathways involved in the rhizome response to desiccation stress. Results indicate that the rhizomes use different molecular mechanisms to achieve desiccation-tolerance in winter and summer. Potential cross-talks and cross-tolerances were identified in which mechanisms protecting the rhizomes against desiccation-tolerance appeared to also protect them against heat stress, and in winter an apparent cross-talk against desiccation and pathogen stresses was also identified. This study is the first report of evidence that M.caffrorum rhizomes are the 'master-regulator organs' responsible for regulating desiccation-tolerance in the fronds. This role was inferred from the rhizome's predicted up-/down-regulation of biological processes and pathways that relate to leaf senescence, shoot system morphogenesis and gametophyte development, among others.en_ZA
dc.identifier.apacitationShoko, R. (2015). <i>A proteomic investigation of the rhizomes of the resurrection fern Mohria caffrorum L. (Desv.) in response to desiccation</i>. (Thesis). University of Cape Town ,Faculty of Humanities ,Department of English Language and Literature. Retrieved from http://hdl.handle.net/11427/16779en_ZA
dc.identifier.chicagocitationShoko, Ryman. <i>"A proteomic investigation of the rhizomes of the resurrection fern Mohria caffrorum L. (Desv.) in response to desiccation."</i> Thesis., University of Cape Town ,Faculty of Humanities ,Department of English Language and Literature, 2015. http://hdl.handle.net/11427/16779en_ZA
dc.identifier.citationShoko, R. 2015. A proteomic investigation of the rhizomes of the resurrection fern Mohria caffrorum L. (Desv.) in response to desiccation. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Shoko, Ryman AB - As there is limited information on the mechanisms of vegetative desiccation-tolerance in resurrection plant rhizomes, this work was undertaken to study the mechanisms of desiccation-tolerance in Mohria caffrorum rhizomes. Fronds of this plant have been previously characterized as being desiccation-tolerant in summer and desiccation-sensitive in winter. Since fern rhizomes are perennial organs, it was of interest to establish whether these organs are also perennially desiccation-tolerant and, whether or not the rhizomes regulate desiccation-tolerance in the fronds. Ultra-structural evidence using transmission electron microscopy and viability studies using electrolyte leakage analysis showed that the rhizomes were desiccation-tolerant throughout the seasons. Quantitative proteomics analysis using isobaric tags for relative and absolute quantification was employed to investigate molecular mechanisms of desiccation-tolerance in the rhizomes of this plant. Using a custom fern rhizome specific peptide sequence database, 236 proteins were identified. Of these, 16 proteins increased in abundance while 14 declined, in the summer collected rhizomes. On the other hand, 16 proteins increased in abundance and 20 declined in the winter form. Western blot analysis confirmed the expression trends of heat shock protein 70-2 and superoxide dismutase-[Cu-Zn], which were among the differentially expressed proteins. Bioinformatics analysis of the differentially expressed proteins was carried out using network enrichment tools, to identify key molecular processes and pathways involved in the rhizome response to desiccation stress. Results indicate that the rhizomes use different molecular mechanisms to achieve desiccation-tolerance in winter and summer. Potential cross-talks and cross-tolerances were identified in which mechanisms protecting the rhizomes against desiccation-tolerance appeared to also protect them against heat stress, and in winter an apparent cross-talk against desiccation and pathogen stresses was also identified. This study is the first report of evidence that M.caffrorum rhizomes are the 'master-regulator organs' responsible for regulating desiccation-tolerance in the fronds. This role was inferred from the rhizome's predicted up-/down-regulation of biological processes and pathways that relate to leaf senescence, shoot system morphogenesis and gametophyte development, among others. DA - 2015 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2015 T1 - A proteomic investigation of the rhizomes of the resurrection fern Mohria caffrorum L. (Desv.) in response to desiccation TI - A proteomic investigation of the rhizomes of the resurrection fern Mohria caffrorum L. (Desv.) in response to desiccation UR - http://hdl.handle.net/11427/16779 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/16779
dc.identifier.vancouvercitationShoko R. A proteomic investigation of the rhizomes of the resurrection fern Mohria caffrorum L. (Desv.) in response to desiccation. [Thesis]. University of Cape Town ,Faculty of Humanities ,Department of English Language and Literature, 2015 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/16779en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of English Language and Literatureen_ZA
dc.publisher.facultyFaculty of Humanitiesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMolecular and Cell Biologyen_ZA
dc.subject.otherresurrection plantsen_ZA
dc.subject.otherDesiccation-toleranceen_ZA
dc.titleA proteomic investigation of the rhizomes of the resurrection fern Mohria caffrorum L. (Desv.) in response to desiccationen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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