Identification of Bacteroides genes involved in Metronidazole resistance

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dc.contributor.advisorAbratt, Valerie Roseen_ZA
dc.contributor.authorCasanueva, Anaen_ZA
dc.date.accessioned2014-07-30T17:36:11Z
dc.date.available2014-07-30T17:36:11Z
dc.date.issued2004en_ZA
dc.descriptionIncludes bibliographical references (leaves [123]-141).
dc.description.abstractBacteroides species are Gram-negative obligate anacrobes that live in the gastrointestinal tract of mammals and are thought to account for approximately 30% of the colonic microbiota. Certain Bacteroides species, such as B. fragilis and to a lesser extent B. thetaiotaomicron, can become opportunistic pathogens and cause severe infection. The antibiotic of choice for treating such infections is metronidazole, a DNA damaging agent. Metronidazole enters the bacterial cell as an inert prodrug, and is activated by cellular reduction into a cytotoxic compound which is thought to cause DNA strand breaks. Certain metronidazole resistant B. fragilis strains have been described, where the drug was not reduced inside the cell due to decreased activity of the metabolic enzymes which are involved in this process. Little is known about the mechanisms involved in repair of metronidazole damage and the potential for resistance. In this study, two difIerent approaches were used to isolate and analyse Bacteroides genes involved in metronidazole resistance, with emphasis on DNA repair genes. These methods were transposon mutagenesis of Bacteroides, and functional complementation of E. coli metronidazole sensitive mutants with genes from B. fragilis.en_ZA
dc.identifier.apacitationCasanueva, A. (2004). <i>Identification of Bacteroides genes involved in Metronidazole resistance</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/4246en_ZA
dc.identifier.chicagocitationCasanueva, Ana. <i>"Identification of Bacteroides genes involved in Metronidazole resistance."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2004. http://hdl.handle.net/11427/4246en_ZA
dc.identifier.citationCasanueva, A. 2004. Identification of Bacteroides genes involved in Metronidazole resistance. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Casanueva, Ana AB - Bacteroides species are Gram-negative obligate anacrobes that live in the gastrointestinal tract of mammals and are thought to account for approximately 30% of the colonic microbiota. Certain Bacteroides species, such as B. fragilis and to a lesser extent B. thetaiotaomicron, can become opportunistic pathogens and cause severe infection. The antibiotic of choice for treating such infections is metronidazole, a DNA damaging agent. Metronidazole enters the bacterial cell as an inert prodrug, and is activated by cellular reduction into a cytotoxic compound which is thought to cause DNA strand breaks. Certain metronidazole resistant B. fragilis strains have been described, where the drug was not reduced inside the cell due to decreased activity of the metabolic enzymes which are involved in this process. Little is known about the mechanisms involved in repair of metronidazole damage and the potential for resistance. In this study, two difIerent approaches were used to isolate and analyse Bacteroides genes involved in metronidazole resistance, with emphasis on DNA repair genes. These methods were transposon mutagenesis of Bacteroides, and functional complementation of E. coli metronidazole sensitive mutants with genes from B. fragilis. DA - 2004 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2004 T1 - Identification of Bacteroides genes involved in Metronidazole resistance TI - Identification of Bacteroides genes involved in Metronidazole resistance UR - http://hdl.handle.net/11427/4246 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/4246
dc.identifier.vancouvercitationCasanueva A. Identification of Bacteroides genes involved in Metronidazole resistance. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2004 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/4246en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleIdentification of Bacteroides genes involved in Metronidazole resistanceen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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