The histone H1 of the sea urchin embryo, partial structures, enzymatic modifactions and developmental programme

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dc.contributor.advisorVon Holt, Clausen_ZA
dc.contributor.advisorStrickland, W Nen_ZA
dc.contributor.authorDe Groot, Petronella Christinaen_ZA
dc.date.accessioned2015-12-08T06:56:45Z
dc.date.available2015-12-08T06:56:45Z
dc.date.issued1982en_ZA
dc.descriptionBibliography: leaves 207-233.en_ZA
dc.description.abstractDevelopmental biology owes a tremendous debt to sea urchins. These animals have proved to be experimental jewels, and since they are distributed abundantly along the Peninsula coastline, they are readily at hand. Sea urchin embryos have been shown to be extremely well suited for analysis of developmental processes at the ultrastructural, biochemical and molecular levels. This project is a study of the very lysine-rich histone fraction or H1-histone fraction of Parechinus angulosus embryo. The first part deals with the characterization and separation of the H1-variants present in the late gastrula embryo. The second part describes the determination of the partial primary structure of the three chromatographically separated H1-fractions: The amino acid composition and sequences of the H1-histone variants are compared to those of H1-histones from other sea urchin embryo species and from various other sources. The third part is a study of the histone variant synthesis program during the early development. [3H] Lysine incorporation into newly synthesized histones was utilized to examine the histone synthetic program. This part also describes the examination of histone acetylation and phosphorylation occurring during the ninth cell cycle of development. Examination of the modification of the different histone variants and modifications of the histone variants at the different cell stages are discussed.en_ZA
dc.identifier.apacitationDe Groot, P. C. (1982). <i>The histone H1 of the sea urchin embryo, partial structures, enzymatic modifactions and developmental programme</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/15667en_ZA
dc.identifier.chicagocitationDe Groot, Petronella Christina. <i>"The histone H1 of the sea urchin embryo, partial structures, enzymatic modifactions and developmental programme."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1982. http://hdl.handle.net/11427/15667en_ZA
dc.identifier.citationDe Groot, P. 1982. The histone H1 of the sea urchin embryo, partial structures, enzymatic modifactions and developmental programme. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - De Groot, Petronella Christina AB - Developmental biology owes a tremendous debt to sea urchins. These animals have proved to be experimental jewels, and since they are distributed abundantly along the Peninsula coastline, they are readily at hand. Sea urchin embryos have been shown to be extremely well suited for analysis of developmental processes at the ultrastructural, biochemical and molecular levels. This project is a study of the very lysine-rich histone fraction or H1-histone fraction of Parechinus angulosus embryo. The first part deals with the characterization and separation of the H1-variants present in the late gastrula embryo. The second part describes the determination of the partial primary structure of the three chromatographically separated H1-fractions: The amino acid composition and sequences of the H1-histone variants are compared to those of H1-histones from other sea urchin embryo species and from various other sources. The third part is a study of the histone variant synthesis program during the early development. [3H] Lysine incorporation into newly synthesized histones was utilized to examine the histone synthetic program. This part also describes the examination of histone acetylation and phosphorylation occurring during the ninth cell cycle of development. Examination of the modification of the different histone variants and modifications of the histone variants at the different cell stages are discussed. DA - 1982 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1982 T1 - The histone H1 of the sea urchin embryo, partial structures, enzymatic modifactions and developmental programme TI - The histone H1 of the sea urchin embryo, partial structures, enzymatic modifactions and developmental programme UR - http://hdl.handle.net/11427/15667 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/15667
dc.identifier.vancouvercitationDe Groot PC. The histone H1 of the sea urchin embryo, partial structures, enzymatic modifactions and developmental programme. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1982 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/15667en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherBiochemistryen_ZA
dc.titleThe histone H1 of the sea urchin embryo, partial structures, enzymatic modifactions and developmental programmeen_ZA
dc.typeMaster Thesis
dc.type.qualificationlevelMasters
dc.type.qualificationnameMScen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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