The histone H1 of the sea urchin embryo, partial structures, enzymatic modifactions and developmental programme
Master Thesis
1982
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University of Cape Town
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Abstract
Developmental biology owes a tremendous debt to sea urchins. These animals have proved to be experimental jewels, and since they are distributed abundantly along the Peninsula coastline, they are readily at hand. Sea urchin embryos have been shown to be extremely well suited for analysis of developmental processes at the ultrastructural, biochemical and molecular levels. This project is a study of the very lysine-rich histone fraction or H1-histone fraction of Parechinus angulosus embryo. The first part deals with the characterization and separation of the H1-variants present in the late gastrula embryo. The second part describes the determination of the partial primary structure of the three chromatographically separated H1-fractions: The amino acid composition and sequences of the H1-histone variants are compared to those of H1-histones from other sea urchin embryo species and from various other sources. The third part is a study of the histone variant synthesis program during the early development. [3H] Lysine incorporation into newly synthesized histones was utilized to examine the histone synthetic program. This part also describes the examination of histone acetylation and phosphorylation occurring during the ninth cell cycle of development. Examination of the modification of the different histone variants and modifications of the histone variants at the different cell stages are discussed.
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Bibliography: leaves 207-233.
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De Groot, P. 1982. The histone H1 of the sea urchin embryo, partial structures, enzymatic modifactions and developmental programme. University of Cape Town.