Characterisation of the human α2(I) procollagen promoter-binding proteins

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dc.contributor.advisorParker, M Iqbalen_ZA
dc.contributor.authorCollins, Malcolm Roberten_ZA
dc.date.accessioned2018-01-30T14:00:25Z
dc.date.available2018-01-30T14:00:25Z
dc.date.issued1993en_ZA
dc.description.abstractIn an attempt to elucidate the transcriptional mechanisms that regulate the expression of the human α2(I) procollagen gene, cis-acting DNA-elements within the proximal promoter were identified and their corresponding trans-acting factors characterised. The fibroblast cell lines used in this study had previously been transformed with either simian virus 40 (SVWI-38) or by γ-radiation (CT-1). The SVWI-38 fibroblasts do not produce any α2(I) collagen chains, whereas the CT-1 cell line produces normal type I collagen. Previous studies suggested that trans-acting factor(s) may be responsible for the inactivation of the α2(I) procollagen gene in SVWI-38 fibroblasts (Parker et. al. (1989) J. Biol. Chem 264, 7147-7152; Parker et. al. (1992) Nucleic Acids Res. 20, 5825-5830). In this study, the SVWI-38 proximal promoter (-350 to +54) was sequenced and shown to be normal, thereby ruling out any possibility that mutations within this region was responsible for inactivation of the gene.en_ZA
dc.identifier.apacitationCollins, M. R. (1993). <i>Characterisation of the human α2(I) procollagen promoter-binding proteins</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology. Retrieved from http://hdl.handle.net/11427/27139en_ZA
dc.identifier.chicagocitationCollins, Malcolm Robert. <i>"Characterisation of the human α2(I) procollagen promoter-binding proteins."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology, 1993. http://hdl.handle.net/11427/27139en_ZA
dc.identifier.citationCollins, M. 1993. Characterisation of the human α2(I) procollagen promoter-binding proteins. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Collins, Malcolm Robert AB - In an attempt to elucidate the transcriptional mechanisms that regulate the expression of the human α2(I) procollagen gene, cis-acting DNA-elements within the proximal promoter were identified and their corresponding trans-acting factors characterised. The fibroblast cell lines used in this study had previously been transformed with either simian virus 40 (SVWI-38) or by γ-radiation (CT-1). The SVWI-38 fibroblasts do not produce any α2(I) collagen chains, whereas the CT-1 cell line produces normal type I collagen. Previous studies suggested that trans-acting factor(s) may be responsible for the inactivation of the α2(I) procollagen gene in SVWI-38 fibroblasts (Parker et. al. (1989) J. Biol. Chem 264, 7147-7152; Parker et. al. (1992) Nucleic Acids Res. 20, 5825-5830). In this study, the SVWI-38 proximal promoter (-350 to +54) was sequenced and shown to be normal, thereby ruling out any possibility that mutations within this region was responsible for inactivation of the gene. DA - 1993 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1993 T1 - Characterisation of the human α2(I) procollagen promoter-binding proteins TI - Characterisation of the human α2(I) procollagen promoter-binding proteins UR - http://hdl.handle.net/11427/27139 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/27139
dc.identifier.vancouvercitationCollins MR. Characterisation of the human α2(I) procollagen promoter-binding proteins. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry & Structural Biology, 1993 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/27139en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Medical Biochemistry and Structural Biology
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherDNA-Binding Proteins - analysisen_ZA
dc.subject.otherProcollagen - analysisen_ZA
dc.subject.otherProtein bindingen_ZA
dc.titleCharacterisation of the human α2(I) procollagen promoter-binding proteinsen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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