Protein purification and cDNA cloning of suGF1 : a sea urchin nuclear DNA-binding factor

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dc.contributor.authorScherer, Sonja Danielaen_ZA
dc.date.accessioned2016-09-25T16:47:09Z
dc.date.available2016-09-25T16:47:09Z
dc.date.issued1998en_ZA
dc.description.abstractThe upstream regulatory regions of numerous genes contain contiguous deoxyguanosine residues (G·C-rich sequences) which have been implicated in the regulation of gene expression, since they may involve alterations in their DNA structure, the binding of G-string factors and in some cases even the displacement of a nucleosome positioned over this area. A poly( dG).poly( de)-binding protein (suGF1) has previously been identified and purified on a small scale from embryonic nuclear extracts of the sea urchin Parechinus angulosus (1, 2). suGF1 binds in vitro to the H1-H4 intergenic region of the early histone gene battery, and the recognition site contains 11 contiguous Gs which are incorporated into a positioned nucleosome core in vitro. suGFI may be a member of a family of Gstring factors which could be involved in the developmental regulation of unrelated genes in various organisms. Prior to the commencement of this project no protein or DNA sequence information was available on the protein. The main objective of this thesis was to obtain the eDNA and the primary amino acid sequence for suGFI. Using this information, additional aims were to determine the developmental distribution of the protein and obtain insight into the molecular basis of the regulatory function of suGF 1 by analysis of the primary protein structure and expression of the eDNA.en_ZA
dc.identifier.apacitationScherer, S. D. (1998). <i>Protein purification and cDNA cloning of suGF1 : a sea urchin nuclear DNA-binding factor</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/21905en_ZA
dc.identifier.chicagocitationScherer, Sonja Daniela. <i>"Protein purification and cDNA cloning of suGF1 : a sea urchin nuclear DNA-binding factor."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1998. http://hdl.handle.net/11427/21905en_ZA
dc.identifier.citationScherer, S. 1998. Protein purification and cDNA cloning of suGF1 : a sea urchin nuclear DNA-binding factor. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Scherer, Sonja Daniela AB - The upstream regulatory regions of numerous genes contain contiguous deoxyguanosine residues (G·C-rich sequences) which have been implicated in the regulation of gene expression, since they may involve alterations in their DNA structure, the binding of G-string factors and in some cases even the displacement of a nucleosome positioned over this area. A poly( dG).poly( de)-binding protein (suGF1) has previously been identified and purified on a small scale from embryonic nuclear extracts of the sea urchin Parechinus angulosus (1, 2). suGF1 binds in vitro to the H1-H4 intergenic region of the early histone gene battery, and the recognition site contains 11 contiguous Gs which are incorporated into a positioned nucleosome core in vitro. suGFI may be a member of a family of Gstring factors which could be involved in the developmental regulation of unrelated genes in various organisms. Prior to the commencement of this project no protein or DNA sequence information was available on the protein. The main objective of this thesis was to obtain the eDNA and the primary amino acid sequence for suGFI. Using this information, additional aims were to determine the developmental distribution of the protein and obtain insight into the molecular basis of the regulatory function of suGF 1 by analysis of the primary protein structure and expression of the eDNA. DA - 1998 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1998 T1 - Protein purification and cDNA cloning of suGF1 : a sea urchin nuclear DNA-binding factor TI - Protein purification and cDNA cloning of suGF1 : a sea urchin nuclear DNA-binding factor UR - http://hdl.handle.net/11427/21905 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21905
dc.identifier.vancouvercitationScherer SD. Protein purification and cDNA cloning of suGF1 : a sea urchin nuclear DNA-binding factor. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1998 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/21905en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMolecular and Cell Biologyen_ZA
dc.titleProtein purification and cDNA cloning of suGF1 : a sea urchin nuclear DNA-binding factoren_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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