Isolation of the aldose reductase gene (XvAld1) from the resurrection plant Xerophyta viscosa, and characterisation of the gene product and transgenic plants expressing the gene

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dc.contributor.advisorThomson, Jennifer Annen_ZA
dc.contributor.advisorFarrant, Jill Men_ZA
dc.contributor.advisorMundree, Sagadevan Gen_ZA
dc.contributor.authorMaredza, Alice Ten_ZA
dc.date.accessioned2014-07-30T17:38:57Z
dc.date.available2014-07-30T17:38:57Z
dc.date.issued2007en_ZA
dc.descriptionIncludes abstract.
dc.descriptionIncludes bibliographical references (leaves 170-209).
dc.description.abstractThe Xerophyta viscosa aldose reductase cDNA (XvAld1) was isolated from a dehydration library. Gene transcripts that are upregulated during stress are normally involved in protection and/ or adaptation, leading to stress tolerance. The genomic organisation of XvAld1 was characterised using Southern blot analysis and DNA sequencing. The results revealed more than one copy of the gene with a complex banding pattern that was partially resolved by sequencing. The sequencing of PCRamplified genomic clones showed that the gene is organised into nine exons and eight introns spanning ~2.9 kb. The observed nucleotide differences between the sequenced clones could reflect polymorphisms between different copies of the gene. An 870-bp clone of the 5′ untranslated region, matching the 5′ leader sequence on the XvAld1 cDNA was analysed for cis-acting response elements. Many of the sequence motifs matched those for hormonal regulation, organ specific expression, dehydration, high or low temperature responses, light and phytochrome responsiveness, wounding, as well as G-box, CAAT and TATA-boxes.en_ZA
dc.identifier.apacitationMaredza, A. T. (2007). <i>Isolation of the aldose reductase gene (XvAld1) from the resurrection plant Xerophyta viscosa, and characterisation of the gene product and transgenic plants expressing the gene</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/4292en_ZA
dc.identifier.chicagocitationMaredza, Alice T. <i>"Isolation of the aldose reductase gene (XvAld1) from the resurrection plant Xerophyta viscosa, and characterisation of the gene product and transgenic plants expressing the gene."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2007. http://hdl.handle.net/11427/4292en_ZA
dc.identifier.citationMaredza, A. 2007. Isolation of the aldose reductase gene (XvAld1) from the resurrection plant Xerophyta viscosa, and characterisation of the gene product and transgenic plants expressing the gene. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Maredza, Alice T AB - The Xerophyta viscosa aldose reductase cDNA (XvAld1) was isolated from a dehydration library. Gene transcripts that are upregulated during stress are normally involved in protection and/ or adaptation, leading to stress tolerance. The genomic organisation of XvAld1 was characterised using Southern blot analysis and DNA sequencing. The results revealed more than one copy of the gene with a complex banding pattern that was partially resolved by sequencing. The sequencing of PCRamplified genomic clones showed that the gene is organised into nine exons and eight introns spanning ~2.9 kb. The observed nucleotide differences between the sequenced clones could reflect polymorphisms between different copies of the gene. An 870-bp clone of the 5′ untranslated region, matching the 5′ leader sequence on the XvAld1 cDNA was analysed for cis-acting response elements. Many of the sequence motifs matched those for hormonal regulation, organ specific expression, dehydration, high or low temperature responses, light and phytochrome responsiveness, wounding, as well as G-box, CAAT and TATA-boxes. DA - 2007 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2007 T1 - Isolation of the aldose reductase gene (XvAld1) from the resurrection plant Xerophyta viscosa, and characterisation of the gene product and transgenic plants expressing the gene TI - Isolation of the aldose reductase gene (XvAld1) from the resurrection plant Xerophyta viscosa, and characterisation of the gene product and transgenic plants expressing the gene UR - http://hdl.handle.net/11427/4292 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/4292
dc.identifier.vancouvercitationMaredza AT. Isolation of the aldose reductase gene (XvAld1) from the resurrection plant Xerophyta viscosa, and characterisation of the gene product and transgenic plants expressing the gene. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2007 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/4292en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherCell Biologyen_ZA
dc.titleIsolation of the aldose reductase gene (XvAld1) from the resurrection plant Xerophyta viscosa, and characterisation of the gene product and transgenic plants expressing the geneen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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