Proteomic studies on patient responses to chemotherapy, radiotherapy and immunotherapy in cancers

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dc.contributor.advisorBlackburn, Jonathan Men_ZA
dc.contributor.authorDuarte, Jessica Da Gamaen_ZA
dc.date.accessioned2016-07-08T10:42:30Z
dc.date.available2016-07-08T10:42:30Z
dc.date.issued2015en_ZA
dc.description.abstractThere is increasing evidence that the aberrant expression of cancer-testis (CT) antigens - a family of ca. 150 proteins that are both autoimmunogenic and mainly restricted to tumours in various types of human cancers - makes them attractive immunotherapy targets, as well as possible cancer diagnostic markers. We carried out a retrospective serological study of primary and secondary autoimmune responses of various cohorts of cancer patients prior to and/or following a variety of distinct treatments (chemotherapy, radiotherapy and immunotherapy), using a large number of archived human serum samples. Our goals were to develop and validate a novel cancer-testis and -associated antigen microarray platform and to then explore its utility and general applicability in the cancer immunology field. In addition, we sought to cross-correlate our protein microarray data from specific cohorts with in vitro T-cell re-stimulation assays for a selected subset of patients. Furthermore, as a means of determining the biological significance of our protein microarray data, we also collected clinical patient data where possible. The underlying hypothesis of our study was that there were measurable differences in autoantibody repertoires towards tumour-specific and -associated antigens between pre- and post-treated cancer patient samples (using various trial therapies), potentially augmented by prior chemo- or radiotherapy, which would correlate with likelihood of response of individual patients to a given therapeutic treatment - including those treatments that aim to generate T-cell responses - and which would also correlate with the nature and extent of individual patient responses to treatment.en_ZA
dc.identifier.apacitationDuarte, J. D. G. (2015). <i>Proteomic studies on patient responses to chemotherapy, radiotherapy and immunotherapy in cancers</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry. Retrieved from http://hdl.handle.net/11427/20261en_ZA
dc.identifier.chicagocitationDuarte, Jessica Da Gama. <i>"Proteomic studies on patient responses to chemotherapy, radiotherapy and immunotherapy in cancers."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry, 2015. http://hdl.handle.net/11427/20261en_ZA
dc.identifier.citationDuarte, J. 2015. Proteomic studies on patient responses to chemotherapy, radiotherapy and immunotherapy in cancers. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Duarte, Jessica Da Gama AB - There is increasing evidence that the aberrant expression of cancer-testis (CT) antigens - a family of ca. 150 proteins that are both autoimmunogenic and mainly restricted to tumours in various types of human cancers - makes them attractive immunotherapy targets, as well as possible cancer diagnostic markers. We carried out a retrospective serological study of primary and secondary autoimmune responses of various cohorts of cancer patients prior to and/or following a variety of distinct treatments (chemotherapy, radiotherapy and immunotherapy), using a large number of archived human serum samples. Our goals were to develop and validate a novel cancer-testis and -associated antigen microarray platform and to then explore its utility and general applicability in the cancer immunology field. In addition, we sought to cross-correlate our protein microarray data from specific cohorts with in vitro T-cell re-stimulation assays for a selected subset of patients. Furthermore, as a means of determining the biological significance of our protein microarray data, we also collected clinical patient data where possible. The underlying hypothesis of our study was that there were measurable differences in autoantibody repertoires towards tumour-specific and -associated antigens between pre- and post-treated cancer patient samples (using various trial therapies), potentially augmented by prior chemo- or radiotherapy, which would correlate with likelihood of response of individual patients to a given therapeutic treatment - including those treatments that aim to generate T-cell responses - and which would also correlate with the nature and extent of individual patient responses to treatment. DA - 2015 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2015 T1 - Proteomic studies on patient responses to chemotherapy, radiotherapy and immunotherapy in cancers TI - Proteomic studies on patient responses to chemotherapy, radiotherapy and immunotherapy in cancers UR - http://hdl.handle.net/11427/20261 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/20261
dc.identifier.vancouvercitationDuarte JDG. Proteomic studies on patient responses to chemotherapy, radiotherapy and immunotherapy in cancers. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry, 2015 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/20261en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Medical Biochemistryen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMedical Biochemistryen_ZA
dc.titleProteomic studies on patient responses to chemotherapy, radiotherapy and immunotherapy in cancersen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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