Non-selective, calcium-permeable channels in the apical membrane of rabbit renal tubules and in the basolateral membrane of human renal tubules : an exploratory patch clamp study

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dc.contributor.advisorIsaacson, Leonen_ZA
dc.contributor.authorSaunders, Jamesen_ZA
dc.date.accessioned2016-02-29T11:55:35Z
dc.date.available2016-02-29T11:55:35Z
dc.date.issued1992en_ZA
dc.descriptionIncludes bibliography.en_ZA
dc.description.abstractThe presence of calcium (Ca) channels has been investigated in the apical membrane of various segments of rabbit renal tubules and in the basolateral membrane of human tubules, using the patch clamp technique. The rabbit tubule segments comprised proximal straight tubules (PST), thick ascending limbs (TAL), distal convoluted tubules (OCT), and cortical collecting ducts (CCD). The human tubule segments could not be identified, but were probably of proximal origin. The luminal surfaces of the individual tubule segments were accessed by perfusing the tubule and inserting the patch pipette through the open end or, more frequently, by tearing open the tubule to allow direct access by a patch electrode. Either Ba (90 mM) or Ca (70 mM) was used in the pipettes. Where possible, channel activity was sought in voltage clamped (30 to -60 m V) excised patches. The data were digitized at l kHz, and filtered (200- 500 Hz) by a six-pole Bessel filter.en_ZA
dc.identifier.apacitationSaunders, J. (1992). <i>Non-selective, calcium-permeable channels in the apical membrane of rabbit renal tubules and in the basolateral membrane of human renal tubules : an exploratory patch clamp study</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,MRC/UCT RU for Exercise and Sport Medicine. Retrieved from http://hdl.handle.net/11427/17308en_ZA
dc.identifier.chicagocitationSaunders, James. <i>"Non-selective, calcium-permeable channels in the apical membrane of rabbit renal tubules and in the basolateral membrane of human renal tubules : an exploratory patch clamp study."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,MRC/UCT RU for Exercise and Sport Medicine, 1992. http://hdl.handle.net/11427/17308en_ZA
dc.identifier.citationSaunders, J. 1992. Non-selective, calcium-permeable channels in the apical membrane of rabbit renal tubules and in the basolateral membrane of human renal tubules : an exploratory patch clamp study. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Saunders, James AB - The presence of calcium (Ca) channels has been investigated in the apical membrane of various segments of rabbit renal tubules and in the basolateral membrane of human tubules, using the patch clamp technique. The rabbit tubule segments comprised proximal straight tubules (PST), thick ascending limbs (TAL), distal convoluted tubules (OCT), and cortical collecting ducts (CCD). The human tubule segments could not be identified, but were probably of proximal origin. The luminal surfaces of the individual tubule segments were accessed by perfusing the tubule and inserting the patch pipette through the open end or, more frequently, by tearing open the tubule to allow direct access by a patch electrode. Either Ba (90 mM) or Ca (70 mM) was used in the pipettes. Where possible, channel activity was sought in voltage clamped (30 to -60 m V) excised patches. The data were digitized at l kHz, and filtered (200- 500 Hz) by a six-pole Bessel filter. DA - 1992 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1992 T1 - Non-selective, calcium-permeable channels in the apical membrane of rabbit renal tubules and in the basolateral membrane of human renal tubules : an exploratory patch clamp study TI - Non-selective, calcium-permeable channels in the apical membrane of rabbit renal tubules and in the basolateral membrane of human renal tubules : an exploratory patch clamp study UR - http://hdl.handle.net/11427/17308 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/17308
dc.identifier.vancouvercitationSaunders J. Non-selective, calcium-permeable channels in the apical membrane of rabbit renal tubules and in the basolateral membrane of human renal tubules : an exploratory patch clamp study. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,MRC/UCT RU for Exercise and Sport Medicine, 1992 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/17308en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentMRC/UCT RU for Exercise and Sport Medicineen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherPhysiologyen_ZA
dc.titleNon-selective, calcium-permeable channels in the apical membrane of rabbit renal tubules and in the basolateral membrane of human renal tubules : an exploratory patch clamp studyen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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