Analysis of genes and enzymes involved in the degradation of hemicellulose and cellulose by Butyrivibrio fibrisolvens H17c
Doctoral Thesis
1992
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University of Cape Town
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Abstract
B. fibrisolvens H17c is a Gram-positive obligate anaerobe which has been found in the rumen of most ruminants. Strains of B. fibrisol vens have been reported to exhibit activity toward cellulosic and hemicellulosic substrates. The aim of this thesis was to screen a genebank of B. fibrisolvens H17c DNA and to isolate genes expressing cellulase and xylanase activity. Two genes encoding β-1-4- glucosidase (BglA) and endo-β-1-4-xylanase (XynB) were cloned in E. coli.
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Bibliography: pages 182-223.
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Lin, L. 1992. Analysis of genes and enzymes involved in the degradation of hemicellulose and cellulose by Butyrivibrio fibrisolvens H17c. University of Cape Town.