Replication fidelity in the mircroevolution of mycobacteria
dc.contributor.advisor | Warner, Digby F | en_ZA |
dc.contributor.advisor | Mizrahi, Valerie | en_ZA |
dc.contributor.author | Ditse, Zanele | en_ZA |
dc.date.accessioned | 2015-12-03T14:10:54Z | |
dc.date.available | 2015-12-03T14:10:54Z | |
dc.date.issued | 2015 | en_ZA |
dc.description.abstract | This thesis aimed to elucidate the structure-function relationships determining the differential fidelities of the dnaE1- and dnaE2-encoded mycobacterial PolIIIα subunits under conditions of genotoxic stress. To this end, the role in DnaE1 intrinsic fidelity of highly conserved PHP domain residues was explored by site-directed replacement of targeted amino acids, resulting in a panel of Mycobacterium smegmatis mutants carrying selected dnaE1 alleles. A complementary approach investigated the contribution of the mycobacterial proofreading DnaQ subunit homolog to the maintenance of DnaE1-dependent replicative fidelity by generating a targeted dnaQ knockout mutant. The third component of this study focused on the inferred role of a highly-conserved N-terminal extension and C-terminal pentapeptide motif in the function of the alternative, error-prone DNA PolIIIα subunit, DnaE2. | en_ZA |
dc.identifier.apacitation | Ditse, Z. (2015). <i>Replication fidelity in the mircroevolution of mycobacteria</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry. Retrieved from http://hdl.handle.net/11427/15541 | en_ZA |
dc.identifier.chicagocitation | Ditse, Zanele. <i>"Replication fidelity in the mircroevolution of mycobacteria."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry, 2015. http://hdl.handle.net/11427/15541 | en_ZA |
dc.identifier.citation | Ditse, Z. 2015. Replication fidelity in the mircroevolution of mycobacteria. University of Cape Town. | en_ZA |
dc.identifier.ris | TY - Thesis / Dissertation AU - Ditse, Zanele AB - This thesis aimed to elucidate the structure-function relationships determining the differential fidelities of the dnaE1- and dnaE2-encoded mycobacterial PolIIIα subunits under conditions of genotoxic stress. To this end, the role in DnaE1 intrinsic fidelity of highly conserved PHP domain residues was explored by site-directed replacement of targeted amino acids, resulting in a panel of Mycobacterium smegmatis mutants carrying selected dnaE1 alleles. A complementary approach investigated the contribution of the mycobacterial proofreading DnaQ subunit homolog to the maintenance of DnaE1-dependent replicative fidelity by generating a targeted dnaQ knockout mutant. The third component of this study focused on the inferred role of a highly-conserved N-terminal extension and C-terminal pentapeptide motif in the function of the alternative, error-prone DNA PolIIIα subunit, DnaE2. DA - 2015 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2015 T1 - Replication fidelity in the mircroevolution of mycobacteria TI - Replication fidelity in the mircroevolution of mycobacteria UR - http://hdl.handle.net/11427/15541 ER - | en_ZA |
dc.identifier.uri | http://hdl.handle.net/11427/15541 | |
dc.identifier.vancouvercitation | Ditse Z. Replication fidelity in the mircroevolution of mycobacteria. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry, 2015 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/15541 | en_ZA |
dc.language.iso | eng | en_ZA |
dc.publisher.department | Division of Medical Biochemistry | en_ZA |
dc.publisher.faculty | Faculty of Health Sciences | en_ZA |
dc.publisher.institution | University of Cape Town | |
dc.subject.other | Medical Microbiology | en_ZA |
dc.title | Replication fidelity in the mircroevolution of mycobacteria | en_ZA |
dc.type | Doctoral Thesis | |
dc.type.qualificationlevel | Doctoral | |
dc.type.qualificationname | PhD | en_ZA |
uct.type.filetype | Text | |
uct.type.filetype | Image | |
uct.type.publication | Research | en_ZA |
uct.type.resource | Thesis | en_ZA |
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