Replication fidelity in the mircroevolution of mycobacteria

dc.contributor.advisorWarner, Digby Fen_ZA
dc.contributor.advisorMizrahi, Valerieen_ZA
dc.contributor.authorDitse, Zaneleen_ZA
dc.date.accessioned2015-12-03T14:10:54Z
dc.date.available2015-12-03T14:10:54Z
dc.date.issued2015en_ZA
dc.description.abstractThis thesis aimed to elucidate the structure-function relationships determining the differential fidelities of the dnaE1- and dnaE2-encoded mycobacterial PolIIIα subunits under conditions of genotoxic stress. To this end, the role in DnaE1 intrinsic fidelity of highly conserved PHP domain residues was explored by site-directed replacement of targeted amino acids, resulting in a panel of Mycobacterium smegmatis mutants carrying selected dnaE1 alleles. A complementary approach investigated the contribution of the mycobacterial proofreading DnaQ subunit homolog to the maintenance of DnaE1-dependent replicative fidelity by generating a targeted dnaQ knockout mutant. The third component of this study focused on the inferred role of a highly-conserved N-terminal extension and C-terminal pentapeptide motif in the function of the alternative, error-prone DNA PolIIIα subunit, DnaE2.en_ZA
dc.identifier.apacitationDitse, Z. (2015). <i>Replication fidelity in the mircroevolution of mycobacteria</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry. Retrieved from http://hdl.handle.net/11427/15541en_ZA
dc.identifier.chicagocitationDitse, Zanele. <i>"Replication fidelity in the mircroevolution of mycobacteria."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry, 2015. http://hdl.handle.net/11427/15541en_ZA
dc.identifier.citationDitse, Z. 2015. Replication fidelity in the mircroevolution of mycobacteria. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Ditse, Zanele AB - This thesis aimed to elucidate the structure-function relationships determining the differential fidelities of the dnaE1- and dnaE2-encoded mycobacterial PolIIIα subunits under conditions of genotoxic stress. To this end, the role in DnaE1 intrinsic fidelity of highly conserved PHP domain residues was explored by site-directed replacement of targeted amino acids, resulting in a panel of Mycobacterium smegmatis mutants carrying selected dnaE1 alleles. A complementary approach investigated the contribution of the mycobacterial proofreading DnaQ subunit homolog to the maintenance of DnaE1-dependent replicative fidelity by generating a targeted dnaQ knockout mutant. The third component of this study focused on the inferred role of a highly-conserved N-terminal extension and C-terminal pentapeptide motif in the function of the alternative, error-prone DNA PolIIIα subunit, DnaE2. DA - 2015 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2015 T1 - Replication fidelity in the mircroevolution of mycobacteria TI - Replication fidelity in the mircroevolution of mycobacteria UR - http://hdl.handle.net/11427/15541 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/15541
dc.identifier.vancouvercitationDitse Z. Replication fidelity in the mircroevolution of mycobacteria. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Division of Medical Biochemistry, 2015 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/15541en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDivision of Medical Biochemistryen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMedical Microbiologyen_ZA
dc.titleReplication fidelity in the mircroevolution of mycobacteriaen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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