Molecular genetic studies of bacteroides fragilis

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dc.contributor.advisorWoods, David Ren_ZA
dc.contributor.authorSouthern, James Arnolden_ZA
dc.date.accessioned2016-09-25T16:47:17Z
dc.date.available2016-09-25T16:47:17Z
dc.date.issued1986en_ZA
dc.descriptionBibliography : pages 234-264.en_ZA
dc.description.abstractSome genetic systems operative in Bacteroides fragilis have been successfully investigated. Firstly the bacteriocin produced by the B.fragilis BF-1 strain was purified and partially characterized. This bacteriocin was found to be cell bound and constitutively produced by the bacteria. The purified bacteriocin was a protein with an apparent Mr of 6400-7200, and was relatively heat stable. The action of the bacteriocin resulted in the lysis of sensitive bacteria. As previous reports indicated that a bacteriocin isolated from the BF-1 strain inhibited the action of RNA-polymerase in-vivo and in-vitro, RNA-polymerase was extracted and partially purified from the bacteriocin sensitive B.fragilis BF-2 strain (described in Appendix 5). This enzyme was essentially similar to that described for other Eubacteriales, but was not inhibited in the in vitro assay by the purified bacteriocin described in this thesis.en_ZA
dc.identifier.apacitationSouthern, J. A. (1986). <i>Molecular genetic studies of bacteroides fragilis</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/21909en_ZA
dc.identifier.chicagocitationSouthern, James Arnold. <i>"Molecular genetic studies of bacteroides fragilis."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1986. http://hdl.handle.net/11427/21909en_ZA
dc.identifier.citationSouthern, J. 1986. Molecular genetic studies of bacteroides fragilis. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Southern, James Arnold AB - Some genetic systems operative in Bacteroides fragilis have been successfully investigated. Firstly the bacteriocin produced by the B.fragilis BF-1 strain was purified and partially characterized. This bacteriocin was found to be cell bound and constitutively produced by the bacteria. The purified bacteriocin was a protein with an apparent Mr of 6400-7200, and was relatively heat stable. The action of the bacteriocin resulted in the lysis of sensitive bacteria. As previous reports indicated that a bacteriocin isolated from the BF-1 strain inhibited the action of RNA-polymerase in-vivo and in-vitro, RNA-polymerase was extracted and partially purified from the bacteriocin sensitive B.fragilis BF-2 strain (described in Appendix 5). This enzyme was essentially similar to that described for other Eubacteriales, but was not inhibited in the in vitro assay by the purified bacteriocin described in this thesis. DA - 1986 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 1986 T1 - Molecular genetic studies of bacteroides fragilis TI - Molecular genetic studies of bacteroides fragilis UR - http://hdl.handle.net/11427/21909 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21909
dc.identifier.vancouvercitationSouthern JA. Molecular genetic studies of bacteroides fragilis. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 1986 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/21909en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMicrobiologyen_ZA
dc.titleMolecular genetic studies of bacteroides fragilisen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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