The transcriptome response of leaves of the resurrection plant, Xerophyta humilis to desiccation

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dc.contributor.advisorIlling, Nicolaen_ZA
dc.contributor.authorShen, Arthur Yen-Hsiangen_ZA
dc.date.accessioned2015-05-28T12:24:46Z
dc.date.available2015-05-28T12:24:46Z
dc.date.issued2014en_ZA
dc.descriptionIncludes bibliographical references.en_ZA
dc.description.abstractIn angiosperms, desiccation tolerance, a genetic trait that enables tissues to survive loss of more than 95% of cellular water is widely observed in the seeds, but is only found in the vegetative tissues of a small group of species known as the resurrection plants. Xerophyta humilis is a small resurrection plant indigenous to Southern Africa. In this study, the hypothesis that vegetative desiccation tolerance is derived from an adaptation of seed desiccation tolerance was tested by characterizing changes in the transcriptome of X. humilis leaves during desiccation. The mRNA transcript abundance of a set of 1680 X. humilis genes was analyzed at 6 different stages of water loss in the leaves of X. humilis. Functional enrichment analysis showed that genes that were down-regulated during desiccation were over-represented with genes involved in photosynthesis, cellular developmental processes, as well as transcription regulator activity. Three distinct clusters of up-regulated genes were identified. The earliest set of up-regulated genes were enriched with genes associated with the turnover of proteins and the simultaneous synthesis of proteins required for protection. Enrichment also included genes associated with lipid body synthesis, as well as the transport of storage proteins to vacuoles. Two groups of late desiccation up-regulated genes were also identified, their expression only increased at later stages of desiccation and remained high in the desiccated leaves.en_ZA
dc.identifier.apacitationShen, A. Y. (2014). <i>The transcriptome response of leaves of the resurrection plant, Xerophyta humilis to desiccation</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology. Retrieved from http://hdl.handle.net/11427/13009en_ZA
dc.identifier.chicagocitationShen, Arthur Yen-Hsiang. <i>"The transcriptome response of leaves of the resurrection plant, Xerophyta humilis to desiccation."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2014. http://hdl.handle.net/11427/13009en_ZA
dc.identifier.citationShen, A. 2014. The transcriptome response of leaves of the resurrection plant, Xerophyta humilis to desiccation. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Shen, Arthur Yen-Hsiang AB - In angiosperms, desiccation tolerance, a genetic trait that enables tissues to survive loss of more than 95% of cellular water is widely observed in the seeds, but is only found in the vegetative tissues of a small group of species known as the resurrection plants. Xerophyta humilis is a small resurrection plant indigenous to Southern Africa. In this study, the hypothesis that vegetative desiccation tolerance is derived from an adaptation of seed desiccation tolerance was tested by characterizing changes in the transcriptome of X. humilis leaves during desiccation. The mRNA transcript abundance of a set of 1680 X. humilis genes was analyzed at 6 different stages of water loss in the leaves of X. humilis. Functional enrichment analysis showed that genes that were down-regulated during desiccation were over-represented with genes involved in photosynthesis, cellular developmental processes, as well as transcription regulator activity. Three distinct clusters of up-regulated genes were identified. The earliest set of up-regulated genes were enriched with genes associated with the turnover of proteins and the simultaneous synthesis of proteins required for protection. Enrichment also included genes associated with lipid body synthesis, as well as the transport of storage proteins to vacuoles. Two groups of late desiccation up-regulated genes were also identified, their expression only increased at later stages of desiccation and remained high in the desiccated leaves. DA - 2014 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2014 T1 - The transcriptome response of leaves of the resurrection plant, Xerophyta humilis to desiccation TI - The transcriptome response of leaves of the resurrection plant, Xerophyta humilis to desiccation UR - http://hdl.handle.net/11427/13009 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/13009
dc.identifier.vancouvercitationShen AY. The transcriptome response of leaves of the resurrection plant, Xerophyta humilis to desiccation. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Molecular and Cell Biology, 2014 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/13009en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMolecular and Cell Biologyen_ZA
dc.titleThe transcriptome response of leaves of the resurrection plant, Xerophyta humilis to desiccationen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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