Comprehensive proteomic profiling of clinically relevant strains of Mycobacterium tuberculosis

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dc.contributor.advisorBlackburn, Jonathanen_ZA
dc.contributor.authorPeters, Julian Sen_ZA
dc.date.accessioned2015-05-28T04:14:52Z
dc.date.available2015-05-28T04:14:52Z
dc.date.issued2014en_ZA
dc.descriptionIncludes bibliographical references.en_ZA
dc.description.abstractTuberculosis is an airborne infectious disease caused by the bacillus known as Mycobacterium tuberculosis. Despite limited genetic variability, Mycobacterium tuberculosis strains exhibit vast discrepancies in phenotypic presentation in terms of virulence, elicited immune response and transmissibility. This study aims to use Mass Spectrometry (MS) tools to quantitatively and qualitatively investigate the total proteome expressed by various epidemiologically significant strains within the Mycobacterium tuberculosis complex (MTBC) as well as a clinically relevant non-tuberculous Mycobacteria (NTM) strain when cultured in vitro. We aim to use the experimental data obtained using discovery mass spectrometry to identify candidate proteins to use in the design of multiple reaction monitoring (MRM) MS experiments for targeted biomarker validation in patient derived biological samples such as sputum. Liquid chromatography mass spectrometry (LC MS/MS) and data capture were carried out using the LTQ Orbitrap Velos. 1D LC was carried out on gel fractionated samples to increase proteome coverage. This allowed a significant increase in the number of protein identifications of up to 80% proteome coverage per strain. Comparative analysis of the datasets was carried out to identify and define the core-proteome expressed across all strains as well as to identify differentially expressed proteins amongst the strains.en_ZA
dc.identifier.apacitationPeters, J. S. (2014). <i>Comprehensive proteomic profiling of clinically relevant strains of Mycobacterium tuberculosis</i>. (Thesis). University of Cape Town ,Faculty of Health Sciences ,Department of Clinical Laboratory Sciences. Retrieved from http://hdl.handle.net/11427/12959en_ZA
dc.identifier.chicagocitationPeters, Julian S. <i>"Comprehensive proteomic profiling of clinically relevant strains of Mycobacterium tuberculosis."</i> Thesis., University of Cape Town ,Faculty of Health Sciences ,Department of Clinical Laboratory Sciences, 2014. http://hdl.handle.net/11427/12959en_ZA
dc.identifier.citationPeters, J. 2014. Comprehensive proteomic profiling of clinically relevant strains of Mycobacterium tuberculosis. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Peters, Julian S AB - Tuberculosis is an airborne infectious disease caused by the bacillus known as Mycobacterium tuberculosis. Despite limited genetic variability, Mycobacterium tuberculosis strains exhibit vast discrepancies in phenotypic presentation in terms of virulence, elicited immune response and transmissibility. This study aims to use Mass Spectrometry (MS) tools to quantitatively and qualitatively investigate the total proteome expressed by various epidemiologically significant strains within the Mycobacterium tuberculosis complex (MTBC) as well as a clinically relevant non-tuberculous Mycobacteria (NTM) strain when cultured in vitro. We aim to use the experimental data obtained using discovery mass spectrometry to identify candidate proteins to use in the design of multiple reaction monitoring (MRM) MS experiments for targeted biomarker validation in patient derived biological samples such as sputum. Liquid chromatography mass spectrometry (LC MS/MS) and data capture were carried out using the LTQ Orbitrap Velos. 1D LC was carried out on gel fractionated samples to increase proteome coverage. This allowed a significant increase in the number of protein identifications of up to 80% proteome coverage per strain. Comparative analysis of the datasets was carried out to identify and define the core-proteome expressed across all strains as well as to identify differentially expressed proteins amongst the strains. DA - 2014 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2014 T1 - Comprehensive proteomic profiling of clinically relevant strains of Mycobacterium tuberculosis TI - Comprehensive proteomic profiling of clinically relevant strains of Mycobacterium tuberculosis UR - http://hdl.handle.net/11427/12959 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/12959
dc.identifier.vancouvercitationPeters JS. Comprehensive proteomic profiling of clinically relevant strains of Mycobacterium tuberculosis. [Thesis]. University of Cape Town ,Faculty of Health Sciences ,Department of Clinical Laboratory Sciences, 2014 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/12959en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Clinical Laboratory Sciencesen_ZA
dc.publisher.facultyFaculty of Health Sciencesen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherMedical Biochemistryen_ZA
dc.titleComprehensive proteomic profiling of clinically relevant strains of Mycobacterium tuberculosisen_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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