Kidney stone rarity in South Africa's black population : investigation of the biochemical and physico-chemical properties of Tamm Horsfall Mucoprotein as a possible contributory factor

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dc.contributor.advisorRodgers, Allenen_ZA
dc.contributor.authorCraig, Tracy-Annen_ZA
dc.date.accessioned2014-09-12T07:07:49Z
dc.date.available2014-09-12T07:07:49Z
dc.date.issued2004en_ZA
dc.descriptionIncludes bibliographical references.en_ZA
dc.description.abstractTamm Horsfall mucoprotein (THP) is a powerful inhibitor of calcium oxalate crystallisation. Since urolithiasis in South African blacks is extremely rare, this study was undertaken to compare the relative inhibitory and biochemical properties of this protein in South African black and white healthy and stone—forn1ing male subjects. THP was isolated by salt precipitation, purified by column chromatography and verified by electrophoresis on SDS-polyacrylamide gels. MALDI-TOF mass spectroscopy was used to determine the molecular weights of the protein from each group and their respective amino acids were analysed. All four THP groups were subjected to tryptic finger printing digestion and matched those of the database. The secondary structures of the four THP proteins were also determined using circular dichroism. The carbohydrate moeities of the four THP groups were analysed for and O-linked oligosaccharides. THP from each of the four groups were subjected to a series of crystallisation experiments using a crossover design in which the protein was added to the urine from which it had been originally isolated, as well as to the urines from the other three groups. This process generated a 4x4 protein-urine grid for investigation of the inhibitory properties of THP in different urine environments. Calcium oxalate crystallization was induced by administration of aqueous sodium oxalate and was monitored Using a Coulter Counter. In another series of experiments 14C-oxalate was used to initiate crystallization. Monitoring in these experiments was achieved by scintillation counter techniques. Calcium oxalate crystal aggregation was investigated in the presence and absence of THP using zeta potential measurements and crystal sedimentation experiments.en_ZA
dc.identifier.apacitationCraig, T. (2004). <i>Kidney stone rarity in South Africa's black population : investigation of the biochemical and physico-chemical properties of Tamm Horsfall Mucoprotein as a possible contributory factor</i>. (Thesis). University of Cape Town ,Faculty of Science ,Department of Chemistry. Retrieved from http://hdl.handle.net/11427/7443en_ZA
dc.identifier.chicagocitationCraig, Tracy-Ann. <i>"Kidney stone rarity in South Africa's black population : investigation of the biochemical and physico-chemical properties of Tamm Horsfall Mucoprotein as a possible contributory factor."</i> Thesis., University of Cape Town ,Faculty of Science ,Department of Chemistry, 2004. http://hdl.handle.net/11427/7443en_ZA
dc.identifier.citationCraig, T. 2004. Kidney stone rarity in South Africa's black population : investigation of the biochemical and physico-chemical properties of Tamm Horsfall Mucoprotein as a possible contributory factor. University of Cape Town.en_ZA
dc.identifier.ris TY - Thesis / Dissertation AU - Craig, Tracy-Ann AB - Tamm Horsfall mucoprotein (THP) is a powerful inhibitor of calcium oxalate crystallisation. Since urolithiasis in South African blacks is extremely rare, this study was undertaken to compare the relative inhibitory and biochemical properties of this protein in South African black and white healthy and stone—forn1ing male subjects. THP was isolated by salt precipitation, purified by column chromatography and verified by electrophoresis on SDS-polyacrylamide gels. MALDI-TOF mass spectroscopy was used to determine the molecular weights of the protein from each group and their respective amino acids were analysed. All four THP groups were subjected to tryptic finger printing digestion and matched those of the database. The secondary structures of the four THP proteins were also determined using circular dichroism. The carbohydrate moeities of the four THP groups were analysed for and O-linked oligosaccharides. THP from each of the four groups were subjected to a series of crystallisation experiments using a crossover design in which the protein was added to the urine from which it had been originally isolated, as well as to the urines from the other three groups. This process generated a 4x4 protein-urine grid for investigation of the inhibitory properties of THP in different urine environments. Calcium oxalate crystallization was induced by administration of aqueous sodium oxalate and was monitored Using a Coulter Counter. In another series of experiments 14C-oxalate was used to initiate crystallization. Monitoring in these experiments was achieved by scintillation counter techniques. Calcium oxalate crystal aggregation was investigated in the presence and absence of THP using zeta potential measurements and crystal sedimentation experiments. DA - 2004 DB - OpenUCT DP - University of Cape Town LK - https://open.uct.ac.za PB - University of Cape Town PY - 2004 T1 - Kidney stone rarity in South Africa's black population : investigation of the biochemical and physico-chemical properties of Tamm Horsfall Mucoprotein as a possible contributory factor TI - Kidney stone rarity in South Africa's black population : investigation of the biochemical and physico-chemical properties of Tamm Horsfall Mucoprotein as a possible contributory factor UR - http://hdl.handle.net/11427/7443 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/7443
dc.identifier.vancouvercitationCraig T. Kidney stone rarity in South Africa's black population : investigation of the biochemical and physico-chemical properties of Tamm Horsfall Mucoprotein as a possible contributory factor. [Thesis]. University of Cape Town ,Faculty of Science ,Department of Chemistry, 2004 [cited yyyy month dd]. Available from: http://hdl.handle.net/11427/7443en_ZA
dc.language.isoengen_ZA
dc.publisher.departmentDepartment of Chemistryen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.subject.otherChemistryen_ZA
dc.titleKidney stone rarity in South Africa's black population : investigation of the biochemical and physico-chemical properties of Tamm Horsfall Mucoprotein as a possible contributory factoren_ZA
dc.typeDoctoral Thesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnamePhDen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceThesisen_ZA
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